摘要
目的:构建可涂布于介入器械的人血管内皮生长因子(VEGF)真核表达载体。方法:根据人VEGFA(NM_001025366)目的序列设计引物制备目的基因及载体,双酶切载体p IRES2-ZsGreen1和目的基因homo VEGFA进行连接后转化入大肠杆菌,构建质粒p IRES2-ZsGreen1-homo-VEGFA并测序鉴定。结果:目的基因VEGFA已连接到载体pIRES2-ZsGreen1,大小约1400 bp,与人VEGFA(NM_001025366)目的序列比对结果一致,未见变异。质粒p IRES2-ZsGreen1-homo-VEGFA构建成功。结论:成功构建重组人血管内皮生长因子真核表达载体,可用于涂布介入器械表面进行下一步实验。
Objective:To construct the eukaryotic expression vector for recombinant vascular endothelial growth factor(VEGF)for coating interventional device.Methods:The primer was designed to prepare the target gene and vector according to the sequence of human VEGFA(NM_001025366),and then was connected and transformed to E.coli after double digestion of homo VEGFA and pIRES2-ZsGreen1 plasmids.Then the vector pIRES2-ZsGreen1-homo-VEGFA was constructed and identified.Results:The target gene,VEGFA,has been connected to the vector pIRES2-ZsGreen1,which was a 1400 bp plasmid.The sequence alignment was consistent with that of human VEGFA(NM_001025366)and no mutation was found.Plasmid pIRES2-ZsGreen1-homo-VEGFA was successfully constructed.Conclusions:The eukaryotic expression vector pIRES2-ZsGreen1-homo-VEGFA is successfully constructed,and can be used to coat interventional device.
作者
张一林
贺迎坤
李天晓
刘刚
胡延忠
崔秀坤
Zhang Yilin;He Yingkun;Li Tianxiao;Liu Gang;Hu Yanzhong;Cui Xiukun(Interventional Therapy Center of Zhengzhou University People's Hospital,Zhengzhou 450003,China;Scientific Research Platform of Henan Provincial People's Hospital,Zhengzhou 450003,China;School of Basic Medical Sciences of Henan University,Kaifeng 475001,China)
出处
《中华介入放射学电子杂志》
2019年第1期40-43,共4页
Chinese Journal of Interventional Radiology：electronic edition
基金
国家自然科学基金项目(81601583)
河南省科技攻关项目(162102310268)
河南省卫生系统出国研修项目(2016054)
关键词
血管内皮生长因子
介入器械
质粒
基因
Vascular endothelial growth factor
Interventional device
Plasmid
Gene
