摘要
目的探讨高迁移率族蛋白B1(HMGB1)、肿瘤坏死因子α(TNF-a)及白介素6(IL-6)血清表达水平与慢性阻塞性疾病(COPD)及COPD所致的肺动脉高压的相关性。方法选取自2015年1月至2016年5月收治的COPD患者96例为研究对象,根据患者患病情况将其分为单纯COPD组(A组,n=56)及COPD合并肺动脉高压组(B组,n=40)。另选取20例健康研究对象作为健康组。采用第1秒用力呼气容积(FEV1)占预计值百分比(FEV1%)、FEV1/用力肺活量百分比(FEV1/FVC%)评价各组研究对象的肺功能;用超声心动图测定肺动脉压力;采用酶联免疫吸附试验检测各组研究对象血清HMGB1、TNF-a、IL-6水平并进行比较,分析血清HMGB1、TNF-a、IL-6水平与肺功能、肺动脉压力的相关性。结果 B组肺动脉压明显高于A组和健康组,组间比较,差异均有统计学意义(P <0. 05); B组的FEV1%及FEV1/FVC%均低于A组和健康组,且A组低于健康组,组间比较,差异均有统计学意义(P <0. 05)。B组的HMGB1、TNF-a及IL-6均高于A组、健康组,且A组上述指标均高于健康组,组间比较,差异均有统计学意义(P <0. 05)。相关性分析结果显示,HMGB1、TNF-a、IL-6与肺动脉压呈正相关,与FEVI%、FEV1/FVC%呈负相关。HMGB1与TNF-a、IL-6呈正相关。结论 HMGB1、TNF-a、IL-6介导了COPD全身炎症反应,这些细胞因子在COPD、肺动脉高压的发生、发展过程起重要作用。
Objective To investigate the correlation between serum expression levels of high mobility group protein B1(HMGB1),tumor necrosis factorα(TNF-a)and interleukin-6(IL-6)with chronic obstructive disease(COPD)and pulmonary hypertension caused by COPD.Methods A retrospective study was performed on 96 cases of patients with COPD who were admitted from January 2015 to May 2016.Patients were divided into the simple COPD group(Group A,n=56)and COPD combined with pulmonary hypertension group(Group B,n=40)according to their disease status.Another 20 healthy volunteers were selected as the health group.The estimated percentage of forced expiratory volume(FEV1)in the first second(FEV1%)and percentage of forced vital capacity(FEV1/FVC%)were used to evaluate the pulmonary function of the subjects in each group.Pulmonary artery pressure was measured by echocardiography.The serum HMGB1,TNF-a and IL-6 levels of the subjects in each group were detected by enzyme-linked immunosorbent assay(elisa)and compared.The correlation between serum HMGB1,TNF-a and IL-6 levels and lung function and pulmonary artery pressure was analyzed.Results Pulmonary artery pressure in Group B was significantly higher than that in Group A and health group(P<0.05).FEV1%and FEV1/FVC%of Group B were lower than those of Group A and health group,and Group A was lower than that of health group(P<0.05).HMGB1,TNF-a and IL-6 of Group B were higher than those of Group A and health group,and the above indexes of Group A were higher than those of health group(P<0.05).The results of correlation analysis showed that HMGB1,TNF-a and IL-6 were positively correlated with pulmonary artery pressure,and negatively correlated with FEVI%and FEV1/FVC%.HMGB1 was positively correlated with TNF-a and IL-6.Conclusion HMGB1,TNF-a and IL-6 mediate COPD systemic inflammatory response.These cytokines play an important role in the development of COPD and pulmonary hypertension.
作者
刘温娟
黄爱霞
鲁立文
LIU Wen-juan;HUANG Ai-xia;LU Li-wen(Department of Respiratory,Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus,Shanghai Fengxian District Central Hospital,Shanghai 201401,China)
出处
《临床军医杂志》
CAS
2019年第1期15-17,20,共4页
Clinical Journal of Medical Officers
基金
上海市奉贤区科学技术委员会科学技术发展基金项目(20141203)
关键词
慢性阻塞性肺疾病
肺动脉高压
高迁移率族蛋白B1
肿瘤坏死因子Α
白介素6
Chronic obstructive pulmonary disease
Pulmonary hypertension
High mobility group protein B1
Tumor necrosis factor-a
Interleukin-6
