摘要
背景:国内外卒中后抑郁动物模型主要以脑卒中叠加抑郁造模方式构建,但其各种造模方式都有其优缺点,在此基础上对卒中后抑郁造模方式进行新的探讨。目的:采用行为学评估及脑组织神经介质检测,探究一种稳定、可复制的卒中后抑郁造模方式。方法:雄性SD大鼠90只,体质量(200±20)g,由西安交通大学动物实验中心提供。(1)将60只大鼠随机分为3组,行30,60,90min的缺血再灌注,24h后观察大鼠存活率,并行神经功能缺损评分及脑梗死体积的评定,确定最佳缺血再灌注的时间点;(2)另将30只大鼠随机分为假手术组、大脑中动脉栓塞组、卒中后抑郁组,于刺激前及刺激的第1,2,3周进行强迫游泳实验、敞箱实验、糖水消耗实验、新奇抑制摄食实验、体质量变化评价大鼠的抑郁行为;运用ELisa法检测脑组织中的5-羟色胺、去甲肾上腺素及脑源性神经营养因子水平。结果与结论:(1)确定了60 min为后续缺血再灌注的时间点;(2)行为学评估显示,与大脑中动脉栓塞组相比,卒中后抑郁大鼠的体质量、糖水消耗实验和敞箱运动垂直及水平运动得分均于第2周开始降低(P <0.05),新奇抑制摄食实验潜伏时间、强迫游泳实验不动时间出现明显升高(P<0.05);(3)Elisa检测显示,与大脑中动脉栓塞组相比,卒中后抑郁组大鼠的5-羟色胺、去甲肾上腺素及脑源性神经营养因子水平均降低(P <0.05);(4)结果说明,采用60 min的缺血再灌注联合慢性不可预知的刺激,建立卒中后抑郁模型,可表现出明显的抑郁状态,同时,模型稳定、存活率高,符合卒中后抑郁的多种临床特征,可能成为研究卒中后抑郁一种新的、可靠的造模方式。
BACKGROUND:Animal models of post-stroke depression are established by stroke combined with depression induction,and each modeling method has its own advantages and shortcomings.A new modeling method is needed.OBJECTIVE:To explore a stable and replicable model of post-stroke depression through behavioral assessment and brain tissue neurotransmitter detection.METHODS:Ninety male Sprague-Dawley rats weighting(200±20)g provided by Laboratory Animal Center of Xi’an Jiaotong University were enrolled.Sixty rats were randomly divided into three groups and then underwent 30,60 and 90 minutes of ischemia/reperfusion.The survival rate of rats was observed after 24 hours,and the scores of neurological function deficit and the volume of cerebral infarct were evaluated.The optimal time points of ischemia/reperfusion were determined.The remaining 30 rats were randomly divided into sham,middle cerebral artery occlusion,and post-stroke depression groups.Forced swimming test,open field test,the novelty-suppressed feeding test,sucrose preference test and body mass change were used to evaluate rat depression behaviors before and 1,2,and 3 weeks after stimulation.The levels of 5-hydroxytryptamine,norepinephrine,and brain-derived neurotrophic factor in brain tissue were detected by ELISA.RESULTS AND CONCLUSION:The optimal time point 60 minutes was determined for subsequent ischemia/reperfusion.Behavioral evaluation showed that compared with the middle cerebral artery occlusion group,body mass,sucrose preference test and the vertical and horizontal movement scores of the open field test began to decrease at 2 weeks in the post-stroke depression group(P<0.05).The latency of the novelty-suppressed feeding and the time of forced swimming test were significantly increased(P<0.05).The results of ELISA showed that 5-hydroxytryptamine,norepinephrine,and brain-derived neurotrophic factor levels in the post-stroke depression group were significantly lower than those in the middle cerebral artery occlusion group(P<0.05).Our findings imply tha
作者
赵兵兵
李涛
王豆
周奇
闫咏梅
Zhao Bingbing;Li Tao;Wang Dou;Zhou Qi;Yan Yongmei(First Clinical Medical College of Shaanxi University of Chinese Medicine,Xianyang 712000,Shaanxi Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第11期1755-1760,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金(81373840)
项目负责人:闫咏梅~~
