摘要
目的探讨微小RNA-26a(miR-26a)对食管癌Eca109细胞增殖、迁移、凋亡和细胞周期的影响。方法将正常培养的人食管鳞状细胞癌Eca109细胞随机分为正常对照组(正常培养的不加任何类型慢病毒的Eca109细胞)、阴性对照组(转染随机序列慢病毒)及miR-26a过表达组(转染miR-26a过表达慢病毒)。分别采用四甲基偶氮唑盐实验、细胞划痕实验、流式细胞术检测细胞增殖、迁移、细胞周期和凋亡情况。结果转染后72、96 h,miR-26a过表达组细胞增殖能力低于正常对照组和阴性对照组(P <0. 05);阴性对照组与正常对照组细胞增殖能力比较差异无统计学意义(P> 0. 05)。转染后72 h,miR-26a过表达组细胞划痕愈合率低于正常对照组和阴性对照组(P <0. 05);阴性对照组与正常对照组细胞划痕愈合率比较差异无统计学意义(P> 0. 05)。转染后48 h,miR-26a过表达组G_1、G_2期细胞所占比例高于正常对照组和阴性对照组,S期细胞所占比例低于正常对照组和阴性对照组(P <0. 05)。阴性对照组与正常对照组细胞G_1、S和G_2期细胞所占比例比较差异无统计意义(P> 0. 05)。结论 miR-26a可以抑制人食管鳞状细胞癌Eca109细胞增殖和迁移,阻断细胞由G_1期向S期过渡,但对细胞的凋亡无明显影响。
Objective To investigate the influence of microRNA-26a(miR-26a)on the proliferation,migration,apoptosis and cell cycle of esophageal cancer cell line Eca109.Methods Human esophageal squamous cell carcinoma cells Eca109 were randomly divided into normal control group(normal cultured Eca109 cells),negative control group(transfected random sequence lentivirus)and miR-26a overexpression group(transfected with lentivirus miR-26a).Methyl thiazolyl tetrazolium assay,cell scratch assay and flow cytometry were used to detect cell proliferation,migration,cell cycle and apoptosis.Results At 72,96 hours after transfection,the proliferation ability of the cells in the miR-26a overexpression group was lower than that in the normal control group and negative control group(P<0.05).There was no significant difference in the proliferation ability between the normal control group and the negative control group(P>0.05).At 72 hours after transfection,the healing rate of the cells in the miR-26a overexpression group was lower than that in the normal control group and the negative control group(P<0.05).There was no significant difference in the healing rate between the normal control group and the negative control group(P>0.05).The proportion of G1 and G2 phase cells in the miR-26a overexpression group was higher than that in the normal control group and the negative control group at 48 h after transfection,the proportion of S phase cells was lower than that in the normal control group and the negative control group(P<0.05).There was no significant difference in the proportion of G1,S and G2 phase between the normal control group and the negative control group(P>0.05).Conclusion miR-26a can inhibit the proliferation and migration of esophageal squamous cell carcinoma Eca109 cells,and block the transition from G1 to S phase,but does not affect the apoptosis of cells.
作者
贺家勇
徐茜
杨晨晨
HE Jia-yong;XU Qian;YANG Chen-chen(Department of Surgery,Staff-worker Hospital,Urumqi Petrochemical Subsidiary of China National Petroleum,Urumqi 830019,Xinjiang Uyghur Autonomous Region,China;Department of Immunology,College of Basic Medicine,Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China;Editorial Department,Journal of Xinjiang Medical University,Urumqi 830011,Xinjiang Uyghur Autonomous Region,China)
出处
《新乡医学院学报》
CAS
2019年第2期131-135,共5页
Journal of Xinxiang Medical University
基金
新疆维吾尔自治区自然科学基金面上项目(编号:2017D01C211)
