摘要
应用高通量测序技术,以鸢尾为研究对象,通过分析其与固定化净水菌剂共同作用处理河道污水过程中水体微生物群落的变化过程,探索植物-微生物的相互作用和耦合效应。实验用水为河道污水,设置鸢尾、鸢尾+固定化净水菌、固定化净水菌和空白对照4个实验组,通过控制流速,在实验装置内进行动态模拟实验。结果显示,相较于进水,各个实验组出水中氨氮呈下降趋势,并在6 d后保持稳定,总氮去除效率较低。水体总有机碳同样呈下降趋势,并在3 d后保持稳定,但化学需氧量去除效率较低。水体细菌多样性丰富,涵盖10个门67个属,细菌群落呈动态变化过程,鸢尾和固定化净水菌均对其产生影响。氨氧化功能微生物主要集中在变形菌门,已知分类的种属相对丰度较低。反硝化功能微生物集中在变形菌门的6个属,群落存在动态变化过程。结果表明,鸢尾与固定化净水菌在水体净化过程中具有显著作用,对水体细菌群落变化产生影响,并存在着一定的协同作用,其中,固定化净水菌的作用强于鸢尾。
In order to explore the plant-microorganism interaction and coupling effects,high throughput sequencing technique was used to study the dynamic changes of microbial community in the river sewage by the coupling of Iris tectorum and immobilized water purification bacteria.The source water used in the experiment was river sewage.Four experimental groups were set up:Iris tectorum,Iris tectorum and immobilized water purification bacteria,immobilized water purification bacteria,and blank control.The dynamic simulation experiment was carried out in the experimental device by controlling the flow rate.The results demonstrated that,compared with the inflow water,the ammonia nitrogen in the outflow was on a downward trend,and remained stable after 6 days.The removal efficiency of total nitrogen was low.Total organic carbon in the water also presented a downturn trend and remained stable after 3 days.The removal efficiency of chemical oxygen demand was low.The water was rich in bacterial diversity,covering 10 phyla and 67 genera.The bacterial communities presented a dynamic changing function,which was influenced both by Iris tectorum and immobilized water purification bacteria.Ammonia oxidizing functional microorganisms were mainly affiliated in Proteobacteria,with rather low relative abundance in the species of known classifications.Denitrification functional microorganisms mainly focused on 6 genera of Proteobacteria,whose communities also presented a dynamic changing process.The results showed that Iris tectorum and immobilized bacteria had a significant effect on the water purification process,and had a certain synergistic effect on the changes of bacterial community in water body,and the effect of immobilized water purification bacteria was stronger than that of Iris tectorum.
作者
刘宗楠
王新
吴逸飞
姚晓红
孙宏
沈琦
李维琳
汤江武
LIU Zongnan;WANG Xin;WU Yifei;YAO Xiaohong;SUN Hong;SHEN Qi;LI Weilin;TANG Jiangwu(College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)
出处
《浙江农业学报》
CSCD
北大核心
2019年第1期121-129,共9页
Acta Agriculturae Zhejiangensis
基金
浙江省重点研发计划(2015C03004)
浙江省公益技术应用研究项目(LGF19C01001)
关键词
水体污染
固定化菌剂
高通量测序
water pollution
immobilized bacteria
high-throughput sequencing
