摘要
为评价复方止痢中药制剂长期用药的安全性,利用其对大鼠进行长期毒性试验,将160只SD大鼠随机分为低、中、高剂量组(分别为6.66、22.20、66.60 g/kg)和对照组(生理盐水20. 00 m L/kg) 4组,每组40只,雌雄各半。保证每天给药1次,每周给药6 d,连续给药6个月。记录大鼠的临床症状和体质量,并分别于给药第3个月和第6个月进行血液学、血清生化指标检测,并进行系统尸检以及脏器系数、组织病理学检查。结果表明,灌胃复方止痢中药制剂后,大鼠均存活,饮水、采食等行为活动表现正常,体质量明显增加;各剂量组血液学及血清生化指标与对照组相比无显著性差异,且停药2周后各指标也均无显著性差异;剖检观测各脏器组织位置、质量和脏器系数均正常,部分脏器质量有所增加,与对照组相比无显著性差异;组织病理学检查结果表明,高剂量组和对照组大鼠未见明显与给药相关的异常病理和毒性改变,恢复期停药后未见延迟性毒性。因此,长期服用复方止痢中药制剂对大鼠未见明确的毒性反应,停药后亦无延迟性毒性反应,说明复方止痢中药制剂具有良好的临床安全性。
In order to evaluate the safety of long-term treatment of compound Chinese medicine preparations in rats,long-term toxicity test was used.160 SD rats were randomly divided into 4 group:low,medium and high dose groups(6.66,22.20,66.60 g/kg)and blank control group(physiological saline 20.00 mL/kg),each group included 40 rats(male∶female=1∶1).Rats were administered compound Chinese medicine preparations once a day for 6 days per week for 6 months.The clinical symptoms and body weight of the rats were recorded.At the 3rd and 6th month,each group was tested for the haematological indexes,biochemical indexes,organ coefficients and histopathological examination.The results showed that the rats survived after the administration of the medicine,and the activities of drinking water and feeding were normal and the body weight increased significantly.The haematological and biochemical indexes of each dose group were not significantly different from the control group,and the same as 2-week restoration.The position,weight and organ coefficient of each organ were normal and the weight of some organs increased.There was no significant difference compared with the control group.The pathological results showed that there were no obviously pathological and toxic changes of drug administration of rats in the compound Chinese medicine preparations high-dose group and the control group,and no delayed toxicity after the withdrawal period.Therefore,the long-term use of compound Chinese medicine preparations has no toxicity in rats,and there is no delayed toxicity after stopping the medicine.The test showed that compound Chinese medicine preparations are safe in clinical practice.
作者
张涛
姜玲玲
唐远江
余波
周思旋
杨何
杨粤黔
冯将
ZHANG Tao;JIANG Lingling;TANG Yuanjiang;YU Bo;ZHOU Sixuan;YANG He;YANG Yueqian;FENG Jiang(Husbandry and Veterinary Research Institute,Guiyang 550005,China;Guizhou Hongyu Animal Husbandry Technology Development Co.,Ltd.,Guiyang 550005,China;Institute of Animal Health Supervision of Jiangkou County,Jiangkou 554400,China)
出处
《河南农业科学》
北大核心
2019年第1期120-127,共8页
Journal of Henan Agricultural Sciences
基金
贵州省重大科技专项(黔科合重大专项字[2013]6014号)
贵州省农业科技攻关项目(黔科合NY[2015]3009-2号)
关键词
复方止痢中药制剂
长期毒性
大鼠
Compound Chinese medicine preparation
Long-term toxicity
Rats
