摘要
【目的】探究不同^(60)Co-γ射线辐射对朝牧1号谷稗种子幼苗生长以及生理效应的影响。【方法】以朝牧1号谷稗种子为试材,采用0、50、100、150、200和250 Gy的^(60)Co-γ射线辐射谷稗种子,对不同辐射剂量的谷稗种子进行幼苗培养,测定辐射对其幼苗的生长和叶片生理生化的影响,并对各指标进行相关性和主成分分析。【结果】辐射抑制了谷稗幼苗的生长,250 Gy对其幼苗的生长抑制作用较强;随着辐射剂量的增加,可溶性糖、脯氨酸、可溶性蛋白和总叶绿素含量均呈先增后减的变化趋势,相对电导率和丙二醛含量呈先减后增的变化趋势,过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性均呈先增后减的变化趋势。【结论】综合主成分的方差贡献率和主成分的原性状相关矩阵值计算出特征向量分析发现,50～200 Gy辐射剂量可用于谷稗的辐射育种。
[Purpose]To investigate the effects of different 60Co-γ-rays irradiation for Echinochloa crusgalli‘Zhaomu No.1’on the growth and physiological effects of seedlings.[Method]The seeds of E.crusgalli‘Zhaomu No.1’were used as the test material and treated with different doses of 60Co-γradiation.60Co-γ-ray irradiation dose was 0,50,100,150,200 and 250 Gy.E.crusgalli seeds were cultured seedlings with different radiation doses.The changes of growth,physiological and biochemical indexes of leaves were measured and the indexes were analyzed by correlation and principal component analysis.[Result]Radiation inhibited the growth of E.crusgalli seedlings,and 250 Gy doses of radiation had a stronger inhibitory effect on their seedlings.With the increase of radiation dose,soluble sugar,proline,soluble protein and total chlorophyll content was increased first and then decreased trend.The relative conductivity and malondialdehyde content decreased first and then increased.Peroxidase(POD)and catalase(CAT)and superoxide dismutase(SOD)showed a trend of increased first and then decreased.[Conclusion]By analyzing the variance contribution ratio of principal components and the correlation matrix value of principal components,the characteristic vector analysis shows that the 50?200 Gy radiation dose can be used in the radiation breeding of E.crusgalli.
作者
李波
邬婷婷
马赫
LI Bo;WU Tingting;MA He(College of Life Science,Agriculture and Forestry,Qiqihar University,Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar 161006,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2019年第1期83-88,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
黑龙江省教育厅基本业务专项(135209267)
黑龙江省应用技术研究与开发计划重大项目(GA15B105-5)
关键词
谷稗
60CO-Γ辐射
幼苗生长
生理指标
相关性分析
主成分分析
Echinochloa crusgalli
60Co-γradiation
germination
physiological index
correlation analysis
principal component analysis
