摘要
目的探讨梅毒螺旋体不同检测方法在新生儿梅毒诊断中的应用价值。方法选择我院2015年1月~2017年12月确诊的45例新生儿梅毒患者和其对应产妇45例及100例同期健康新生儿作为研究对象,分别采用梅毒螺旋体酶联免疫吸附试验(TP-ELISA)、密螺旋体颗粒凝集实验(TPPA)和甲苯胺红不加热血清试验(TRUST)对所有研究对象血清进行检测,比较三种检测方法对新生儿梅毒的诊断价值。结果梅毒产妇TRUST试验各血清抗体滴度下梅毒的检测阳性率普遍高于新生儿(100.00%vs. 53.33%,P<0.05)。45例梅毒产妇的三种检测结果均为阳性,新生儿TRUST检测阳性检出率明显低于产妇,差异有统计学意义(53.33%vs. 100.00%,P<0.05)。新生儿TPELISA检测阳性检出率和TPPA检测阳性检出率与产妇比较,差异无统计学意义(97.78%vs. 100.00%,100.00%vs. 100.00%,P>0.05)。通过对45例梅毒新生儿和100例健康新生儿血清检测,结果显示,TRUST检测出24例梅毒阳性,TP-ELISA检测出44例梅毒阳性,TPPA检测出100例梅毒阳性,TPPA检测的灵敏度(100.00%)和特异度(100.00%)均明显高于TRUST检测(53.33%,91.00%)和TP-ELISA检测(97.78%,96.00%)。结论在新生儿梅毒的诊断中,TP-ELISA检测的灵敏度和特异度均较高,适合作为新生儿梅毒的初筛实验,而TPPA检测过程相对繁琐,可作为确诊实验。
Objective To explore the value of different methods of Treponema pallidum(TP) in the diagnosis of neonatal syphilis. Methods A total of 45 neonatal syphilis patients, 45 cases of corresponding puerpera and 100 healthy neonates diagnosed in our hospital from January 2015 to December 2017 were selected as study subjects. They were detection with Treponema pallidum enzyme-linked immunosorbent assay(TP-ELISA), Treponema pallidum particles agglutination assay(TPPA) and toluidine red unheated serum test(TRUST). The diagnostic value of three detection methods for neonatal syphilis were compared. Results The positive rate of syphilis detected by TRUST test in all serum antibody titers of syphilis parturients was generally higher than that of newborns(100.00% vs. 53.33%, P<0.05). Positive results were found in all 45 TP parturients by these three methods, the positive rate of TRUST in TP newborns was significantly lower than that in TP parturients,with significant difference(53.33% vs. 100.00%, P<0.05). There was no significant difference of positive results in TP-ELISA and TPPA between TP newborns and TP parturients(97.78% vs. 100.00%,100.00%vs. 100.00%, P>0.05). After serum check for 45 TP newborns and 100 healthy newborns, it showed that 24 cases of positive results were found by TRUST, 44 cases of positive results were found by TP-ELISA and 100 cases of positive results were found by TPPA. The sensitivity(100.00%) and specificity(100.00%) of TPPA were significantly higher than those of TRUST(53.33%, 91.00%) and TP-ELISA(97.78%, 96.00%)(P<0.05). Conclusion In the diagnosis of neonatal syphilis, the sensitivity and specificity of TP-ELISA are high, which is suitable for the initial screening test of neonatal syphilis. The detection process of TPPA is relatively complicated and can be used as a confirmatory test.
作者
罗杏
LUO Xing(Department of Clinical Laboratory,People′s Hospital of Xingning City in Guangdong Province,Xingning 514500,China)
出处
《中国当代医药》
2018年第36期143-145,共3页
China Modern Medicine
关键词
新生儿梅毒
梅毒螺旋体
检验
诊断
灵敏度
Neonatal syphilis
Treponema pallidum
Test
Diagnosis
Sensitivity
