摘要
目的探讨丹曲胶囊对心肌缺血再灌注损伤的线粒体保护机制。方法将健康雄性SD大鼠30只按随机数字表法分为五组,每组6只,分别为假手术组、模型组、丹曲胶囊300 mg/kg组、丹曲胶囊600 mg/kg组、单硝酸异山梨酯组(10 mg/kg)。采用左冠状动脉前降支结扎的方法造模,分别记录缺血/再灌注不同时间点ST段变化,采用酶联免疫吸附测定(ELISA)法检测心肌损伤标志物[心肌肌钙蛋白T(cTnT)、肌酸激酶同工酶(CK-MB)]水平,并用透射电镜观察心肌线粒体超微结构,用免疫印迹法测定线粒体分裂蛋白p-Drp1以及融合蛋白Mfn2的表达。结果丹曲胶囊300、600 mg/kg组均能够降低心肌损伤标志物水平(P <0.01),明显缓解心电图ST段上抬(P <0.01),并能够减轻心肌细胞线粒体分裂,使线粒体分裂蛋白p-Drp1的表达减少(P <0.01),而线粒体融合蛋白Mfn2表达差异无统计学意义(P> 0.05)。结论丹曲胶囊能够减轻心肌缺血/再灌注损伤,改善心肌缺血状态,减少心肌细胞线粒体的分裂,可能与其抑制线粒体分裂蛋白p-Drp1的表达相关。
Objective To explore the mitochondrial protective mechanism of Danqu Capsules on myocardial ischemia/reperfusion injury. Methods Thirty healthy male SD rats were divided into 5 groups by random number table method(n = 6):sham operation group, model group, 300 mg/kg of Danqu Capsules group, 600 mg/kg of Danqu Capsules group, and Isosorbide Mononitrate group(10 mg/kg). The method of ligation of the left anterior descending branch of coronary artery was used to make model, and the changes of ST segment at different time points of ischemia/reperfusion were recorded.The levels of myocardial injury markers [cardiac troponin T(cTnT), creatine kinase isoenzyme(CK-MB)] were measured by enzyme-linked immunosorbent assay(ELISA) method. The ultrastructure of myocardial mitochondria was observed with transmission electron microscope, and the expression of mitochondrial fission protein p-Drp1 and fussion protein Mfn2 was measured by immunoblotting. Results 300, 600 mg/kg of Danqu Capsules groups could reduce the levels of myocardial injury markers(P < 0.01), relieve the ST segment elevating of ECG(P < 0.01), reduce mitochondrial fission of myocardial cells and lessen the expression of mitochondrial protein p-Drp1(P < 0.01), but there was no significant difference in the expression of mitochondrial fusion protein Mfn2(P > 0.05). Conclusion Danqu Capsules can alleviate myocardial ischemia/reperfusion injury, improve myocardial ischemia and reduce mitochondrial fission of myocardial cells, which may be related to the inhibition of the expression of mitochondrial fission protein p-Drp1.
作者
田心
徐攀
刘超峰
TIAN Xin;XU Pan;LIU Chaofeng(Department of Cardiology,Traditional Chinese Medical Hospital of Shaanxi Province,Shaanxi Province,Xi′an 710003,China;Department of Clinical Medicine,Hubei University of Medicine,Hubei Province,Shiyan 442000,China)
出处
《中国医药导报》
CAS
2018年第36期16-19,共4页
China Medical Herald
基金
陕西省中医药管理局中医药科研课题(2017JCM S009)
陕西省科技统筹创新工程计划项目(2016KTZDSF01-03-02)
关键词
丹曲胶囊
心肌缺血/再灌注损伤
线粒体
保护机制
Danqu Capsules
Myocardial ischemia/reperfusion injury
Mitochondria
Protective mechanism
