摘要
目的:分离纯化得到金钗石斛多糖,考察其对过氧化氢(H2O2)诱导肝癌细胞株(Hep G2)细胞损伤模型的抗衰老活性。方法:以金钗石斛为原料,经脱脂、提取、浓缩、乙醇沉淀和真空干燥后,得到金钗石斛多糖,然后经二乙氨乙基(DEAE-52)纤维素柱水洗纯化获纯化金钗石斛多糖DNPL。检测金钗石斛多糖DNPL的中性糖含量,应用高效凝胶渗透色谱(HPGPC)对其相对分子质量进行分析,同时研究了金钗石斛多糖对H2O2诱导Hep G2细胞损伤模型的抗衰老活性。结果:金钗石斛粗多糖的中性糖含量为68. 58%,相对分子质量为2. 8×104Da,金钗石斛多糖(DNPL)对H2O2诱导的肝癌细胞有明显的保护作用,随浓度的升高抗氧化作用增强,并且在浓度高于500μg·m L-1后强于阳性参照CAPE的抗氧化作用。结论:选用H2O2诱导Hep G2细胞损伤模型,结果显示金钗石斛多糖具有较显著的抗细胞衰老作用。
Objective: To isolate and purify polysaccharides from Dendrobium nobile,and study the effect of H2 O2 on HepG2 cell injury induced by anti-aging activity. Methods: The D. nobile polysaccharides were obtained by degreasing, extraction, concentration, ethanol precipitation and vacuum drying. The polysaccharide of D. obile were purified by DEAE-52 cellulose column. The neutral sugar content of polysaccharide of D. nobile was determined. The relative molecular mass of D. nobile polysaccharide was detected by high performance gel permeation chromatography( HPGPC). The anti-aging activity of HepG2 cells induced by hydrogen peroxide was also studied. Results: The crude sugar content of D. nobile polysaccharide was 68. 58 % and the relative molecular mass was 2. 8 × 104 Da. D. nobile polysaccharide has obvious protective effect on hepatoma cells induced by H2 O2, and the antioxidant effect was enhanced with the increase of concentration, and it was stronger than that of positive reference CAPE after the concentration was higher than 500 μg·mL-1. Conclusion: This experiment based on model of HepG2 cells induced by H2 O2 to study the antioxidant activity of polysaccharides, indicates that D. nobile polysaccharide has the obvious resistance to cell aging.
作者
谢苗苗
肖柳
杨磊
杨全伟
XIE Miao-miao;XIAO Liu;YANG Lei;YANG Quan-wei(Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430000,China;The First Hospital of Hunan University of Chinese Medicine,Changsha 410000,China;Wuhan No.1 Hospital,Wuhan 430000,China)
出处
《中国现代中药》
CAS
2018年第12期1489-1493,共5页
Modern Chinese Medicine
基金
湖北省自然科学基金面上项目(2014CFH396)
关键词
金钗石斛
多糖
分离纯化
抗衰老
Dendrobium nobile
polysaccharide
separation and purification
anti-aging
