摘要
目的 COI基因对柴达木盆地部分蚤种进行分子生物学鉴定,旨在完善蚤类传统形态分类法的不足。方法PCR扩增68份蚤类标本的COI基因片段(约600bp)并进行测序和比对;用MEGA 6软件的K2-P双参数模型计算种内及种间遗传距离,以邻接法(neighbor-joining,NJ)构建系统发育树。结果共测得柴达木盆地19种蚤的68条COI基因序列,种内遗传距离0.01%～2.9%,种间遗传距离3%～15.4%,种间遗传距离显著大于种内遗传距离。结论 NJ树显示同一物种形成高支持率的单系,种间分支很明显,表明COI可以作为DNA条形编码基因对柴达木盆地的常见蚤类进行物种鉴定。
In order to making up the deficiency of the traditional morphological classification method,we were being to identify fleas of the molecular biology by DNA barcoding in the study.The partial fragments(about 600 bp)of the mt DNA COI gene were amplified from 68 samples of fleas by PCR,and the obtained COI gene fragments were sequenced and aligned.The intra-and inter-species genetic distances were calculated among the closely-related species with neighbor-joining(NJ)method.We got 68 COI gene sequences and calculated the genetic distance about 19 kinds of fleas that the intraspecific distance 0.01%-2.9%,interspecific distance 3%-15.4%,interspecific genetic distance was significantly greater than the intraspecific genetic distance.NJ tree showed the same species have formed a single line with high support rates and interspecific branch was clearly.The result showed COI gene appear to be accurate and efficient markers for fleas identification.
作者
李海龙
何建
赵延梅
杨汉青
马英
LI Hai-Long;HE Jian;ZHAO Yan-Mei;YANG Han-Qing;MA Ying(Qinghai Institute for Endemic Disease Prevention and Control,Xining 811602,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2018年第12期1107-1111,共5页
Chinese Journal of Zoonoses
基金
国家自然科学基金项目(No.81560521)
青海省基础研究计划项目(No.2016-ZJ-770)
青海省鼠疫防控与研究重点实验室(No.2017-ZJ-Y22)联合资助~~
