摘要
利用RACE技术从文心兰花瓣中克隆到RR基因,将其命名为OnRR9(登录号:MH758789)。OnRR9基因全长为627 bp,编码208个氨基酸。对其进行蛋白质2级结构分析,构建进化树,并进行实时荧光定量分析。结果表明:OnRR9蛋白为亲水性的分泌蛋白,定位于细胞核中。同源序列比对显示,OnRR9基因属于Yes N/Ara C家族,氨基酸序列中含有保守的REC结构域,可能参与双组分信号转导途径。构建进化树表明,OnRR9蛋白与拟南芥中A型ARRs亲缘关系接近,OnRR9基因可能作为细胞分裂素信号传导的成分。实时荧光定量分析结果表明,OnRR9基因在盛开期前后表达模式发生逆转,表明它可能参与文心兰花瓣衰老启动过程。
A gene of response regulator,OnRR9 gene(GenBank accession number:MH758789),was cloned from the petals of Oncidium Gower Ramsey flowers with RACE technology.The OnRR9 gene is 627 bp in length and encodes a protein of 208 amino acids in size.The secondary structure analysis of the protein showed that OnRR9 protein is a hydrophilic secreted protein localized in the nucleus.The homologous sequence alignment revealed that the OnRR9 gene belongs to the YesN/AraC family and contains a conserved REC domain in the amino acid sequence,which may be involved in the two-component signal transduction pathway.The construction of the phylogenetic tree demonstrated that the OnRR9 protein is closely related to the type A ARRs in Arabidopsis thaliana,suggesting that the OnRR9 may be involved in the response of cytokinin signaling.Real-time fluorescence quantitative analysis indicated that the OnRR9 gene had the maximum expression in the full blossom flower but was low expressed before and after full blossom,suggesting that this gene might play a role in the iniitation of petal senescence of Oncidium Gower Ramsey flowers.
作者
闫冰玉
巩笑笑
谭玉荣
王鹏
李双江
潘英文
刘进平
YAN Bingyu;GONG Xiaoxiao;TAN Yurong;WANG Peng;LI Shuangjiang;PAN Yingwen;LIU Jinping(Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources,Institute of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China;Post-Entry Quarantine Station for Tropical Plant,Hainan Entry-Exit Inspection and Quarantine Bureau,Haikou,Hainan 570311,China)
出处
《热带生物学报》
2018年第4期393-400,共8页
Journal of Tropical Biology
基金
国家自然科学基金项目(31560573)
海南大学热带农林学院作物学科研究生科研创新课题(ZWCX2018030)
海南大学作物学优秀本科生人才培养计划课题(ZWCX2018046)
