摘要
【目的】优化红缘拟层孔菌多糖的超声提取工艺,为其工业化生产和综合利用提供依据。【方法】以红缘拟层孔菌为材料,考察超声功率、提取温度、液(mL)料(g)比和提取时间对红缘拟层孔菌多糖得率的影响;在此基础上,通过Box-Behnken响应面法,设计4因素3水平试验,建立多糖提取回归方程,确定其最佳提取工艺。【结果】超声功率、提取温度、液(mL)料(g)比和提取时间4个因素对红缘拟层孔菌多糖提取的影响大小依次为:提取温度>超声功率>提取时间>液料比,在单因素试验及响应面优化基础上,确定红缘拟层孔菌多糖的最佳提取工艺为:超声功率60W,提取温度63℃,液料比21∶1,提取时间32min;在此条件下,多糖得率为12.87%,纯度为69.42%。【结论】得到了红缘拟层孔菌多糖超声提取的优化工艺,该工艺方便可行。
【Objective】Ultrasonic extraction of polysaccharides from Fomitopsis pinicola was optimized to provide basis for its development and application.【Method】The effects of ultrasonic power extraction,temperature,liquid(mL)-solid(g)ratio and extraction time on yield of F.pinicola polysaccharide were investigated.Then,experiments were designed based on Box-Behnken response surface with 4 factors and 3 levels,and the regression equation of polysaccharide extraction was established to determine the optimum extraction conditions.【Result】The effects of the 4 factors on the extraction of polysaccharides from red marginal bacteria were in the order of ultrasonic temperature>power extraction>extraction time>liquid(mL)-solid(g)ratio.On the basis of single factor test and response surface optimization,the optimum extraction conditions for polysaccharide extraction from red border were ultrasonic power 60 W,extraction temperature 63℃,solid to liquid ratio 21∶1,and extraction time 32 min.The obtained yield of polysaccharides was 12.87%and the purity was 69.42%.【Conclusion】The optimal ultrasonic extraction technology for polysaccharides from F.pinicola was convenient and feasible.
作者
王玉梅
WANG Yumei(Department of Traditional Chinese Medicine and Pharmacology,Medical College,Hexi University,Zhangye,Gansu 734000,China)
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2019年第1期116-122,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(81660759)
关键词
红缘拟层孔菌
多糖
超声提取
Fomitopsis pinicola
polysaccharides
ultrasonic extraction
