摘要
ATS(algalturfscrubber)系统是一种利用潮间带藻类高效去除富营养化水体中氮(N)、磷(P)元素的水质净化系统,藻类是支持整个系统最重要的组成部分,其群落结构与水质净化效果密切相关。该研究基于Illumina MiSeq平台的高通量测序技术对海水水族缸的ATS系统中藻板样品和自然礁石上的藻类进行多样性分析。18S rDNA研究结果表明, ATS系统中经筛选得到藻类优化序列21390条,其中对丝藻属Antithamnion占主要地位,占序列总数的22.79%,其次19.07%序列归入斜纹藻属Psammodictyon;宋井、展南亭、后宅3个地区的藻类经筛选分别得到88435、87129、106458条优化序列,主要为分枝色指藻属Chroodactylon、宽果藻属Mastocarpus和日本马泽藻属Mazzaella。ATS系统和自然环境样品共有的优势藻为骨条藻属Skeletonema和仙菜属Ceramium。本实验在研究ATS系统与自然礁石上的藻类群落相似性的同时,深入探究了其群落结构的差异性,为进一步优化ATS系统的功能提供了理论依据。
Algal turf scrubber(ATS) is a system for water cleaning, which uses intertidal algae to effectively eliminate nitrogen(N) and phosphorus(P) elements in eutrophic water. Algae is the most important organism in the whole system, and its community structure is closely related to the effect of water purification. Based on the high-throughput sequencing technology of Illumina MiSeq platform, we analyze the diversity of algal samples in ATS system and natural reefs in this study. The results of 18 S rDNA show that the ATS system obtains 21390 algal majorizing sequences. Among these majorizing sequences, Antithamnion accounts for 22.79% of the total number, and the rest sequences are classified as Psammodictyon, accounting for 19.07%. Algae in Song Jing, Zhan Nanting and Hou Zhai areas have 88435, 87129 and 106458 majorizing sequences after filtering, respectively. They are mainly Chroodactylon, Mastocarpus and Mazzaella. Both the ATS system and the natural environment samples have the dominant species of Skeletonema and Ceramium. This research shows that the ATS system has similarity and specificity in its community structure compared to the natural reefs, which provides a theoretical evidence for further improving the function of the ATS system.
作者
王欢欢
谭斯尹
王慧
杜虹
侯涛
陈贤建
黎俊珺
黄莹
WANG Huanhuan;TAN Siyin;WANG Hui;DU Hong;HOU Tao;CHEN Xianjian;LI Junjun;HUANG Ying(Biology department,Shantou University,STU-UNIVPM Joint Algal Research Center,Shantou 515063,China)
出处
《热带海洋学报》
CAS
CSCD
北大核心
2019年第1期43-48,共6页
Journal of Tropical Oceanography
基金
广东大学生科技创新培育专项资金(pdjh2017a0195)~~
关键词
ATS
系统
高通量测序
藻类多样性
潮间带藻
ATS system
high-throughput sequencing
algal diversity
intertidal algal
