摘要
以天然氨基酸为手性原料合成了4种新型的手性方酰胺荧光探针分子(5～8),该合成路线简短,后处理简单,无需柱层析纯化.以荧光光谱为检测手段,测试了此类探针分子对苯丙氨酸、缬氨酸和脯氨酸的手性识别效果,结果表明,探针分子5可以有效识别苯丙氨酸的2个对映异构体.当加入L-苯丙氨酸后,探针分子5的荧光强度显著增强,而加入D-苯丙氨酸后探针分子5的荧光强度显著减弱,2种异构体的荧光强度比(I_L/I_D)可达2. 4.
Four novel squaramide fluorescence probes were synthesised using natural amino acids as chiral sources,which featuers concise synthetic route,simple work-up and purification without column chromatography.The chiral recognition effects of these squaramide probes for phenylalanine,valine and proline were tested using fluorescence spectrum,which indicated that probe 5 could effectively discriminate two enantiomers of phenylalanine.The fluorescence intensity of probe 5 was remarkably enhanced after adding L-phenylalanine;on the contrary,it was observably reduced when D-phenylalanine was added,and the fluorescence intensity ratio of two enantiomers(IL/ID)was up to 2.4.
作者
白蕾
霍淑慧
陈晶
卢小泉
BAI Lei;HUO Shuhui;CHEN Jing;LU Xiaoquan(College of Chemistry and Chemical Engineering,Northwest Normal University,Key Laboratory of Bioelectrochemistry &Environmental Analysis of Gansu Province,Lanzhou 730070,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2019年第1期41-46,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:21575115
21462036)
教育部长江学者与创新研究团队计划项目(批准号:IRT1283)
甘肃省自然科学基金(批准号:18JR3RA085)资助~~
关键词
手性识别
荧光光谱
方酰胺
Α-氨基酸
Chiral recognition
Fluorescence spectrum
Squaramide
α-Amino acid
