摘要
目的:探讨单次链脲佐菌素(STZ)腹腔注射制备糖尿病裸鼠模型的适宜浓度。方法:选取雄性裸鼠60只,随机分成5个试验组和对照组。腹腔注射不同剂量的STZ诱导糖尿病裸鼠模型。注射后3d, 7d,14d,21d对裸鼠体重、血糖进行测量,21d处死动物对胰腺进行组织病理学观察及免疫组化染色。结果:注射剂量为150mg/kg及180mg/kg STZ组裸鼠成模;对照组、70mg/kg组、100mg/kg组及200mg/kg组未成模。结论:通过腹腔一次性注射STZ 150mg/kg及180mg/kg STZ均可有效建立糖尿病裸鼠模型,为该模型的应用提供实验基础。
Objective: To investigate the appropriate dose of Streptozotocin for establishing the diabetic nude mice model. Methods: Sixty male nude mice were ransomly divided into control group and model groups. Different doses of STZ were injected into the nude mice to induce the diabetic nude models. The changes of the body weight and blood glucose were measures at 3th, 7th, 14and 21th day after injection. Pancreas of the mice were stained with eosin-hematoxylin staining and immumohistochemical staining. Results: The nude mice were diabetic after 150mg/kg and 180mg/kg STZ administration. The remaining groups were nondiabetic. Conclusion: The dose of 150mg/kg and 180mg/kg STZ administration could be used in nude mice to establish the diabetic model.
作者
王涵
薛彬
史建峰
韩倩倩
王春仁
WANG Han;XUE Bin;SHI Jian-feng;HAN Qian-qian;WANG Chun-ren(National Institute for Food and Drug Control ,Beijing 102629)
出处
《中国医疗器械信息》
2018年第23期33-35,共3页
China Medical Device Information
基金
国家重点研发计划课题"组织诱导型生物材料及产品安全有效性评价关键技术研究"(项目编号:2016YFC1103202)
中国食品药品检定研究院中青年发展研究基金(项目编号:2016C4)
