摘要
ACBP基因的编码产物是细胞内重要的长链酰基辅酶A酯转运蛋白,近年在普通奶牛中发现该基因与泌乳性状关系密切,但是在水牛中罕有报道。为了在水牛中探究ACBP基因的序列及基因表达特征,以揭示其功能。采用RT-PCR测序法对槟榔江水牛ACBP基因的编码区序列进行了克隆,进一步对其进行了功能生物信息学和表达谱分析。结果表明,槟榔江水牛ACBP基因完整编码区序列长264 bp,其编码蛋白是具有87个氨基酸残基的多肽,拥有一个ACBP超家族的功能结构域,包含4个磷酸化位点;该蛋白无信号肽和跨膜结构,是位于胞内的亲水蛋白。氨基酸序列同源性分析显示,槟榔江水牛与牛亚科物种聚在一起,揭示它们ACBP基因功能的相似性。在检测的13个水牛组织中,ACBP基因在非泌乳期高表达于瘤胃、心脏、脾脏组织中,在泌乳期高表达于瘤胃、大脑和心脏中。此外,该基因在泌乳期乳腺、小肠、肝脏中表达,而在非泌乳期这些组织中不表达。水牛与其他物种的ACBP具有相近的氨基酸组成和结构特征,可能参与长链酰基辅酶A酯的转运,在乳脂合成中发挥重要作用。
The coding product of ACBP gene is an important transport protein for long chain acyl coenzyme A esters in cells.Recently,it is found that this gene is closely related to lactation traits in common dairy cows,but it is rarely reported in water buffalo.The purpose of this study is to explore the sequence and gene expression characteristics of buffalo ACBP gene in order to reveal its function.The coding region of ACBP gene of Binglangjiang buffalo was cloned by RT-PCR method,and the gene was then subjected to analysis of functional bioinformatics and gene expression profiling.The sequence of complete coding region of ACBP gene in Binglangjiang buffalo was264bp in length which encoded a protein composed of87amino acid residues.This protein had a ACBP functional domain and contained4phosphorylated sites.It had no signal peptide and transmembrane region and was a hydrophilic protein located in the cell.The homology analysis of ACBP protein showed that Binglangjiang buffalo and the species of bovine subfamily were clustered together,which revealed the functional similarity of their ACBP gene.In the13tissues assayed,buffalo ACBP gene was highly expressed in the rumen,brain,heart during lactating stage and this gene was highly expressed in the rumen,heart,spleen during non-lactating stage.In addtition,ACBP gene was expressed in mammary gland,small intestine and liver during lactating stage,however it was not expressed in those tissues during non-lactating stage.The ACBP of buffalo and other species had similar amino acid composition and structural characteristics,which might participate in the transport of long chain acyl coenzyme A esters and plays an important role in the synthesis of milk fat.
作者
盛丹
刘琴
滕晓红
张永云
吉丽
苗永旺
SHENG Dan;LIU Qin;TENG Xiaohong;ZHANG Yongyun;JI Li;MIAO Yongwang(Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China;Teaching Demonstration Center of the Basic Experiments of Agricultural Majors,Yunnan Agricultural University,Kunming 650201,China;Institute of Animal Husbandry,Yunnan Vocational and Technical College of Agriculture,Kunming 650212,China)
出处
《华北农学报》
CSCD
北大核心
2018年第6期72-79,共8页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31460582
31760659)
云南省应用基础研究重点项目(2014FA032
2007C0003Z)
关键词
槟榔江水牛
ACBP基因
生物信息学分析
理化特征
基因表达谱
Binglangjiang buffalo
ACBP gene
Bioinformatics analysis
Physicochemical properties
Gene expression profile
