摘要
为探索不同分割区段烟叶烘烤中主要生理变化趋势和差异,将烟叶去除主脉等分为叶基(T1)、叶中(T2)、叶尖(T3)3个区段,与整叶(CK)装在同一温湿度自控电热烤箱进行烘烤,测定不同处理叶片含水量、色素和主要酶活性变化差异。结果表明,不同区段处理含水量主要在42～47℃差异较大,42℃末为CK>T1>T2>T3,47℃末为CK>T2>T3>T1;叶绿素含量在开烤至47℃末T1较高;类胡萝卜素含量在开烤至42℃末T1较高;类叶比在47℃末差异最大,且T3>T2>CK>T1;淀粉酶活性均在38℃末达到第1个峰值,且T3>T2>T1>CK;在38～54℃末CK的多酚氧化酶(PPO)活性最大,T1、T2、T3间无明显差异;各处理过氧化物酶(POD)活性均在38℃末达到最高峰,CK、T2、T3较接近,T1最低。在烘烤过程中烟叶不同区段叶内含水量、色素、酶活性变化趋势大体相似,但在不同烘烤关键阶段存在一定差异,这为优化片烟烘烤工艺和提高烤后烟叶质量提供了理论依据。
In order to explore the difference and trends of the main physiological changes in different sections of tobacco leaves during curing, tobacco leaves with midrib removed were divided into three sections: leaf base(T1), middle leaf(T2), and leaf tip(T3). After that, they were cured with the whole leaf(CK) in the same temperature and humidity in an auto-controlled electric flue-curing oven to determine the changes of moisture content, pigments and main enzymes activities in different treatments. The results showed that the moisture content of different treatments varied greatly from 42 to 47 ℃. At the end of 42 ℃, CK>T1>T2>T3, while at the end of 47 ℃, CK>T2>T3>T1; chlorophyll content was higher in T1 from the beginning of curing to the end of 47 ℃, and carotenoid content was higher in T1 from the beginning of curing to the end of 42 ℃; the difference of carotenoid/chlorophyll ratio was the largest at the end of 47 ℃, showing T3>T2>CK>T1; amylase activity reached the first peak at the end of 38 ℃, and T3>T2>T1>CK; polyphenol oxidase(PPO) activity was higher at CK from 38 to 54 ℃, and there was no significant difference between T1, T2 and T3; peroxidase(POD) activity reached the peak at the end of 38 ℃, CK, T2, T3 were close, and T1 was the lowest. In the process of flue-curing, the changes of moisture content, pigments and enzymes activities in different sections were roughly similar, but there were certain differences at different key stages of flue-curing, which provided a theoretical basis for optimizing flue-curing technology and improving the quality of flue-cured tobacco leaves.
作者
张烨
王松峰
许锡祥
高峻
张建强
刘辉
高宪辉
孙福山
ZHANG Ye;WANG Songfeng;XU Xixiang;GAO Jun;ZHANG Jianqiang;LIU Hui;GAO Xianhui;SUN Fushan(Tobacco Research Institute of CAAS,Key Laboratory of Tobacco Biology and Processing,Ministry of Agriculture,Qingdao 266101,China;Graduate School of Chinese Academy of Agricultural Science,Beijing 100081,China;Sanming Tobacco Company of Fujian Province,Sanming,Fujian 365000,China;Liangshan Branch of Sichuan Tobacco Co.,Xichang,Sichuan 615000,China;China Tobacco Jiangsu Industrial Co.,Ltd.,Nanjing 210019;Yunnan Tobacco Leaf Co.,Ltd.,Kunming 650218,China)
出处
《中国烟草科学》
CSCD
北大核心
2018年第6期66-72,共7页
Chinese Tobacco Science
基金
中国农业科学院科技创新工程(ASTIP-TRIC03)
四川省烟草公司凉山州公司项目"异常烟叶烘烤及上部叶一次采烤关键技术优化与应用"(201751340024080)
四川省烟草公司攀枝花市公司科技项目"攀枝花烟叶质量特质挖掘与彰显关键技术研究与应用"(201517)。
关键词
烤烟
烘烤
不同区段
生理指标
flue-cured tobacco
flue-curing
different sections
physiological index
