摘要
目的分析高尿酸血症患者与正常对照者尿液外泌体源性microRNA表达谱的差异,为mi-croRNA在高尿酸血症发病机制中的研究提供依据。方法用高通量测序法检测3例高尿酸血症患者和3例正常对照者尿液外泌体源性microRNA表达谱,选择部分差异表达的microRNA通过实时荧光定量PCR验证高通量测序结果的可靠性。结果高尿酸血症组与正常对照组相比差异表达的已知microRNA共10种(P <0.05),其中1种表达上调,9种表达下调;并发现差异表达的新microRNA 2种(P <0.05),均表达下调。实时荧光定量PCR结果与测序结果并不完全一致,说明测序结果可供参考,但仍需实时荧光定量PCR进一步验证。结论高尿酸血症组尿液外泌体源性microRNA表达谱与正常对照组有明显差异,该差异表达的microRNA可能参与了高尿酸血症的发生发展,并有望成为诊断高尿酸血症的新型无创生物标志物。
Objective To study differences of microRNA expression profiles in the urinary exosomes be-tween hyperuricemia patients and healthy volunteers and provide the evidences for the pathogenesis of hyperurice-mia.Methods The high-throughput sequencing microRNA was used to analyze the microRNA expression pro-files,and validation of the results was carried out by real-time quantitative PCR(qPCR).Results Compared with the control group,ten known microRNA were expressed differently and significantly(P<0.05),including one up-regulated microRNA and nine down-regulated microRNA in the hyperuricemia patients.In addition,two novel mature microRNA were expressed differently and significantly(P<0.05),which were lower in the hyperuri-cemia.The results of qPCR were not consistent with the sequencing results,which demonstrated that the sequenc-ing results were available for the reference and qPCR was needed for further validation.Conclusion The microR-NA expression profiles are different between hyperuricemia patients and healthy volunteers,and the different ex-pression of microRNA may be involved in the occurrence and development of hyperuricemia.
作者
胡艳艳
徐红
朱仁杰
杨汝春
陈坚翱
HU Yanyan;XU Hong;ZHU Renjie;YANG Ruchun;CHEN Jianao(Jiaxing Hospital of Traditional Chinese Medicine Affiliated to Zhejiang University of Traditional Chinese Medicine,Jiaxing 314001,China)
出处
《实用医学杂志》
CAS
北大核心
2018年第23期3921-3923,3928,共4页
The Journal of Practical Medicine
基金
浙江省科技计划项目(编号:2017C33196)
