摘要
利用直接浓缩并结合液-液萃取从白酒中分离纯化出一种三肽,并通过高效液相色谱-四极杆-飞行时间质谱联用仪进行鉴定,确定其序列为Arg-Asn-His(RNH),质量浓度为(18.78±0.34)μg/L。采用合成的RNH标准品研究其对2,2’-偶氮双(2-甲基丙脒)二盐酸盐(2,2’-azobis(2-methyl propionamidine) dihydrochloride,AAPH)诱导的人体肝癌HepG2细胞氧化损伤的保护作用。结果表明,RNH对细胞内活性氧具有清除作用,其作用是通过增加细胞内抗氧化酶(过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶)的活力及细胞内还原性化合物谷胱甘肽浓度来完成的,同时其可以防止丙二醛含量的增加,降低细胞中AAPH诱导的脂质过氧化水平,从而提高细胞的抗氧化能力,且其抗氧化能力呈浓度依赖性增加。本研究结果能帮助人们更好地认识白酒与健康之间的关系。
In this paper,a novel tripeptide was isolated at a concentration of(18.78±0.34)μg/L from Chinese Baijiu by direct condensation,and its structure was identified as Arg-Asn-His(RNH)by high performance liquid chromatographquadrupole-time-of-flight mass spectrometry.The synthetic RNH was used to explore the protective effect of RNH on oxidative damage induced by2,2’-azobis(2-methyl propionamidine)dihydrochloride(AAPH)in human hepatoma cells(HepG2).The results showed that RNH had a potent scavenging effect against intracellular reactive oxygen species by increasing the activities of antioxidant enzymes(catalase,superoxide dismutase and glutathione peroxidase)and the level of reduced glutathione in the cells.In addition,it prevented the increase of malondialdehyde level and decreased AAPH-induced lipid peroxidation level,thereby enhancing cellular antioxidant defense capacity.The antioxidant activity of the peptide was dose dependent.The results of this study can provide better understanding of the relationship between Chinese Baijiu and health.
作者
霍嘉颖
孙宝国
郑福平
孙金沅
孙啸涛
李贺贺
罗雪莲
黄明泉
HUO Jiaying;SUN Baoguo;ZHENG Fuping;SUN Jinyuan;SUN Xiaotao;LI Hehe;LUO Xuelian;HUANG Mingquan(Beijing Advanced Innovation Centre for Food Nutrition and Human Health, State Key Laboratory of Light Industrial Liquor Qualityand Safety, School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China;National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第23期126-133,共8页
Food Science
基金
“十三五”国家重点研发计划重点专项(2016YFD0400500)
国家自然科学基金面上项目(31471665)
国家自然科学基金青年科学基金项目(31701567).
关键词
白酒
多肽
高效液相色谱-四极杆-飞行时间质谱
抗氧化活性
人肝癌HEPG2细胞
Chinese Baijiu
peptide
high performance liquid chromatograph-quadrupole-time-of-flight mass spectrometry
antioxidant activity
HepG2cell
