摘要
目的:观察推拿对2型糖尿病(Type 2 Diabetes Mellitus,T2DM)大鼠腹直肌组织腺苷酸活化蛋白激酶_(α2)(AMPK_(α2))、核因子-κB(Nuclear Factor-κB,NF-κB)及白细胞介素-6(Interleukin-6,IL-6)基因转录及蛋白表达的影响,探讨其改善T2DM大鼠代谢性炎性损伤的分子机制。方法:选取40只Wistar大鼠,随机取出6只为空白组,其余高脂高糖饮食联合低剂量(35 mg/kg)链脲佐菌素腹腔注射建立T2DM模型,成模大鼠32只随机分入模型组、西药组、推拿组及西药推拿组。空白组及模型组每日灌胃蒸馏水,西药组每天以250 mg/kg标准进行二甲双胍灌胃1次,推拿组每日给予推拿手法治疗1次,西药推拿组在相同给药标准基础上每日加用推拿治疗1次。共干预8周,分别记录第2、4、8周的空腹血糖(Fast Blood Glucose,FBG)值,并运用realtime-PCR测定腹直肌AMPK_(α2)、NF-κB及IL-6基因转录,Western-blot测定腹直肌AMPKα2及NF-κB蛋白含量,ELISA测定IL-6蛋白含量。结果:与模型组比较,西药组及西药推拿组FBG在第2、4、8周时显著下降,差异有统计学意义(P <0. 05,P <0. 01),推拿组FBG在第4、8周时显著下降,差异有统计学意义(P <0. 05);与西药组比较,西药推拿组在第4周降糖效果更为显著,差异有统计学意义(P <0. 05);与推拿组比较,西药推拿组在第4、8周时降糖效果更为显著,差异有统计学意义(P <0. 05);与0周时比较,各观察组FBG在第2、4、8周时,均显著降低,差异有统计学意义(P <0. 05,P <0. 01)。与空白组比较,模型组AMPK_(α2)mRNA转录及蛋白表达均下降,差异有统计学意义(P <0. 05);与模型组比较,西药组、推拿组、西药推拿组AMPK_(α2)mRNA转录及蛋白表达均升高,差异有统计学意义(P <0. 05)。与空白组比较,模型组NF-κBmRNA转录显著升高,差异有统计学意义(P <0. 01),蛋白表达升高,差异有统计学意义(P <0. 05);与模型组比较,西药组NF-κBmRNA转录下降,差异有统计学意义(P <0. 05);与�
Objective:To observe the effects of Tuina on the gene transcription and protein expression of 5′AMP-activated protein kinaseα2(AMPKα2),nuclear factor-κB(NF-κB)and interleukin(IL-6)in rectus abdominis muscle of type 2 Diabetes Mellitus(T2DM)rats.To explore the molecular mechanism of Tuina on improving the metabolic inflammatory injury in T2DM.Methods:A total of 6 Wistar rats out of 40 were randomly selected as the blank group(CON).Others were feed with high-fat and high-sugar diets combined with low-dose(35 mg/kg)streptozotocin to establish a T2DM model.A total of 32 successfully modeled rats were randomly divided into the model group(MOD),western medicine group(MET),and Tuina group(TNA)and western medicine combined Tuina group(MIX).The CON and the MOD were perfused with distilled water daily.The MET was daily given a dose of 250 mg/kg for metformin,and the TNA was given daily for manual treatment,and the MIX was given both intervention from MET and TNA.After 8 weeks of intervention,the values of fast blood glucose(FBG)at 2 nd,4 th,and 8 th week were recorded,and real-time PCR was used to measure the gene transcription of AMPKα2,NF-κB,and IL-6 in the rectus abdominis.Western-blot was used to measure the protein expression of AMPKα2 and NF-κB in the rectus abdominis muscle,and the protein expression of IL-6 was determined by ELISA.Results:Compared with the MOD,the FBG of the MET and the MIX decreased significantly at the 2 nd,4 th,and 8 th week(P<0.05,P<0.01),and the FBG of the TNA decreased significantly at the 4th and 8th week(P<0.05).Compared with the MET,the MIX had a more significant hypoglycemic effect at the 4th week(P<0.05),and compared with the TNA,the MIX had a more significant hypoglycemic effect at the 4th and 8th week(P<0.05).Compared with 0 weeks,FBG of each treatment group was significantly decreased at the 2 nd,4 th,and 8 th week(P<0.05,P<0.01).Compared with the CON,the mRNA and protein expression of AMPKα2 in the MOD was decreased(P<0.05).Compared with the MOD,the mRNA and protein expres
作者
王康
孟凤仙
钮妍
薛恬珏
国生
沈潜
魏培栋
付国兵
Wang Kang;Meng Fengxian;Niu Yan;Xue Tianjue;Guo Sheng;Shen Qian;Wei Peidong;Fu Guobing(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China;;Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《世界中医药》
CAS
2018年第12期3108-3113,共6页
World Chinese Medicine
基金
国家自然科学基金面上项目(81574092)
北京中医药大学青年教师项目(2017-jms-js-104)
