摘要
目的探讨羧肽酶E(CPE)在胃癌组织中表达及其临床意义。方法选择2010年1月至2011年12月在焦作煤业集团责任有限公司中央医院择期行手术治疗的胃癌患者76例,留取胃癌及癌旁正常组织,免疫组织化学检测胃癌及癌旁正常组织中CPE蛋白表达,实时荧光定量聚合酶链反应检测胃癌及癌旁正常组织中CPE mRNA表达,利用Kaplan-Meier法对胃癌组织中CPE表达与患者生存时间的关系进行分析。结果胃癌组织和癌旁正常组织中CPE蛋白阳性表达率分别为80. 3%(61/76)和36. 8%(28/76),胃癌组织中CPE蛋白阳性表达率高于癌旁正常组织(χ~2=37. 112,P <0. 05)。胃癌组织和癌旁正常组织中CPE mRNA相对表达量分别为2. 07±0. 18和1. 39±0. 14,胃癌组织中CPE mRNA相对表达量显著高于癌旁正常组织(t=25. 981,P <0. 05)。胃癌组织中CPE mRNA相对表达量与TNM分期、淋巴结转移、浆膜浸润、血管侵犯、远处转移有关(P <0. 05)。Kaplan-Meier生存分析显示,低表达组患者平均生存时间为(48. 9±3. 6)个月,总生存率为57. 9%;高表达组患者平均生存时间为(34. 7±2. 7)个月,总生存率为35. 1%;低表达组患者总生存率高于高表达组(χ~2=4. 171,P <0. 05)。结论胃癌组织中CPE呈高表达,其与胃癌发生、发展及不良预后有关,可作为判定患者预后的潜在指标。
Objective To investigate the expression of carboxypeptidase E(CPE)in gastric cancer tissues and its clinical significance.Methods Seventy-six patients with gastric cancer undergoing surgical treatment were selected from January 2010 to December 2011 from Jiaozuo Coal Industry Group Co.,Ltd.Central Hospital.The gastric cancer tissues and adjacent tissues of the patients were selected.The expressions of CPE proteins in gastric cancer and adjacent tissues were detected by using immunohistochemistry.The expressions of CPE mRNA in gastric cancer and adjacent tissues were detected by using real-time fluorescence quantitative polymerase chain reaction.The relationship between the expression of CPE in gastric cancer tissues and the survival time of patients was analyzed by Kaplan-Meier method.Results The positive rate of CPE protein in gastric cancer tissues and the adjacent tissues was 80.3%(61/76)and 36.8%(28/76).The positive expression rate of CPE protein in gastric cancer tissues was significantly higher than that in the adjacent tissues(χ2=37.112,P<0.05).The relative expression level of CPE mRNA in gastric cancer tissues and the adjacent tissues was 2.07±0.18 and 1.39±0.14.The relative expression level of CPE mRNA in gastric cancer tissues was significantly higher than that in the adjacent tissues(t=25.981,P<0.05).The relative expression of CPE mRNA in gastric cancer tissues was correlated with TNM stage,lymph node metastasis,serous invasion,vascular invasion and distant metastasis(P<0.05).Kaplan-Meier survival analysis showed that the average survival time of patients in low expression group was(48.9±3.6)months,the overall survival rate was 57.9%,the average survival time in high expression group was(34.7±2.7)months,the overall survival rate was 35.1%,the average survival time of patients in the low expression group was longer than that in high expression group(χ^2=4.171,P<0.05).Conclusion The expression of CPE in gastric cancer tissues is high.It is related to the clinical process and poor prognosis of gastr
作者
李晶
张慧敏
李书良
LI Jing;ZHANG Hui-min;LI Shu-liang(Department of Gastroenterology,Jiaozuo Coal Industry Group Co.,Ltd.Central Hospital,Jiaozuo 454000,Henan Province,China)
出处
《新乡医学院学报》
CAS
2018年第12期1111-1114,共4页
Journal of Xinxiang Medical University
关键词
胃癌
羧肽酶E
表达
预后
gastric cancer
carboxypeptidase E
expression
prognosis
