摘要
目的通过STR侧翼序列单核苷酸多态性(SNPs)的筛选,构建解决特殊亲缘关系及二人混合样品的个人识别鉴定的新方法。方法筛选STR侧翼序列中的SNPs,对包含SNPs的片段进行扩增并测序获得其分型结果,设计序列特异性引物进行扩增并测序。结果获得该STR位点不同来源个体的分型结果。结论成功构建基于STR侧翼序列SNPs的二人混合样品的检测方法,并可应用于特殊的亲缘关系鉴定中。
Objective A new method was developed for the identification of specific kinships and two-person mixed samples through the screening of X-STR flanking-region single nucleotide polymorphisms(SNPs).Methods The STR flanking region was screened for SNPs.The fragments containing SNPs were then amplified using sequence-specific primers and sequenced for STR locus typing.Results The typing results of the STR locus from different individuals were obtained.Conclusion A detection method based on STR flanking region SNPs was successfully developed for mixed samples containing DNA from two individuals and could be applicable for the identification of kinship.
作者
孙溢华
邢佳鑫
宣金锋
姚军
夏皙
王保捷
SUN Yihua;XING Jiaxin;XUAN Jinfeng;YAO Jun;XIA Xi;WANG Baojie(Department of Forensic Genetics,School of Forensic Medicine,China Medical University,Shenyang 110122,China;Wuzhong Public Security Sub-Bureau,Suzhou Public Security Bureau,Suzhou 215104,China)
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2018年第12期1072-1076,共5页
Journal of China Medical University
基金
国家自然科学基金(81671872)
辽宁省自然科学基金(20170541019)
关键词
法医物证学
X-STR
单核苷酸多态性
混合样品
亲缘鉴定
forensic genetics
X-STR
single nucleotide polymorphism
mixed samples
kinship identification
