摘要
采用鳗鱼带肉骨作为原料,原料预处理后用胰蛋白酶水解法生产富含钙的复合氨基酸液及粉料。研究了酶解的最佳工艺条件,实验结果表明:酶解的最佳条件是在pH为8.0、温度为45℃、加酶量为2%、酶解时间为3h的条件下进行实验。利用紫外分光光度法在215nm和225nm的差值下测定产品中蛋白质的含量,用石墨炉原子吸收法测定产品中钙的含量,产品中蛋白质含量可达29.7%,钙的含量为4.79%。该方法工艺简单,产品可以作为食品鲜味剂原料,也可以作为高级酱油原料,同时对废物进行利用,减少企业环境污染。
In this paper,calcium-rich compound amino acid solution and powder are prepared by trypsin hydrolysis method after pretreatment of eels with fleshy bone as extraction material.Subject to study the optimum process conditions of enzymatic hydrolysis,the experimental results show that the optimum conditions of enzyme solution are pH of 8.0,temperature of 45℃,enzyme additive amount of 2%,enzymatic time of 3 h.Using UV spectrophotometry at 215 nm and 225 nm to determine the protein content in the product,the calcium content in the product is determined by graphite furnace atomic absorption method,the protein content in the product is 29.7%,the calcium content is 4.79%.The method is simple,and the product can be used as the raw material of food flavor enhancer,or as the raw material of advanced soy sauce,it can also reduce corporate environmental pollution by waste utilization.
作者
廖晓峰
于荣
LIAO Xiao-feng;YU Rong(College of Chemical and Biological Materials,East China University of Technology, Nanchang 330038,China;College of Water and Environmental Engineering, East China University of Technology,Nanchang 330038,China)
出处
《中国调味品》
CAS
北大核心
2018年第12期18-22,共5页
China Condiment
关键词
鳗鱼带肉骨
蛋白钙
紫外分光光度法
石墨炉原子吸收分光光度法
eel with fleshy bone
protein calcium
ultraviolet spectrophotometry
graphite furnace atomic absorption spectrometry
