摘要
为了探讨内蒙古地区2型糖尿病患者危险因素与脂联素基因rs266729多态性交互作用,设立病例组来源于内蒙古医科大学附院以及内蒙古中蒙医院糖尿病患者共111例,对照组来源于内蒙古医科大学附属医院体检中心共106例,运用PCR-RFLP技术检测脂联素(ADIPOQ)基因rs266729位点多态性,采用二元logistic回归方法分析脂联素基因rs266729多态性与T2DM的相关性,并且与环境因素进行交互作用分析。结果显示:ADIPOQ基因rs266729多态性等位基因频率分布符合Hardy-Weinberg定律; T2DM基因型频率分布依次为CC(85. 61%)、CG(5. 42%)、GG(9. 02%)。校正身体质量指数(BMI)之后,CG基因型人群是CC基因型人群患T2DM的0. 260倍,且有显著性意义(P=0. 009),而GG基因型人群是CC基因型人群患T2DM的1. 146倍,无显著性意义(P=0. 790)。交互作用分析结果表明:rs266729位点与空腹血糖存在正交互作用;与收缩压、空腹C肽、空腹胰岛素、生活环境存在负交互作用。脂联素基因rs266729多态性可以增加个体2型糖尿病罹患风险,且rs266729基因多态性与空腹血糖、吸烟、酗酒、日常运动、日常情绪、生活紧张度、腰臀比、超重、糖尿病家族史这几项存在正交互作用,与收缩压、空腹C肽、空腹胰岛素、生活环境存在负交互作用。
The interaction was explored between risk factors of adiponectin gene rs 266729 polymorphism and type 2 diabetes mellitus in Inner Mongolia.A total of 111 cases of diabetic patients from the Affiliated Hospital of Inner Mongolia Medical University and Inner Mongolia Hospital of Chinese Medicine were included in the case group.The control group was from 106 cases of the physical examination center of the Affiliated Hospital of Inner Mongolia Medical University.The rs 266729 polymorphism of adiponectin(ADIPOQ)gene was detected by PCR-RFLP.A binary logistic regression analysis was used to analyze the association between the adiponectin gene rs 266729 polymorphism and T2DM,and interact with environmental factors.Results show that the frequency distribution of the rs 266729 polymorphism alleles of the ADIPOQ gene was in accordance with Hardy-Weinberg s law;the frequency distribution of the T2DM genotype was CC(85.61%),CG(5.42%),GG(9.02%).After adjusting for body mass index(BMI),the CG genotype population was 0.260 times higher than that of T2DM in the CC genotype population(P=0.009),whereas the GG genotype population was 1.136 times higher than the CC genotype population in T2DM,with no significant significance(P=0.790).The results of interaction analysis showed that there was a positive interaction between rs 266729 locus and fasting blood glucose,and a negative interaction with systolic blood pressure,fasting C-peptide,fasting insulin,and living environment.The adiponectin gene rs 266729 polymorphism can increase the risk of type 2 diabetes in individuals with rs 266729 polymorphism and fasting blood glucose,smoking,alcoholism,daily exercise,daily mood,life tension,waist-to-hip ratio,overweight,the family history of diabetes has positive interactions with negative interactions with systolic blood pressure,fasting C-peptide,fasting insulin,and living conditions.
作者
崔萌
毕力夫
高玉敏
刘艳超
庞慧
赵灵燕
CUI Meng;BI Li-fu;GAO Yu-min;LIU Yan-chao;PANG Hui;ZHAO Ling-yan(Molecular Epidemiology Laboratory of Chronic Diseases,School of Public Health,Inner Mongolia Medical University,Hohhot 010110,China;Department of Epidemiology,School of Public Health,Inner Mongolia Medical University,Hohhot 010110,China;Medicine Experimental Center, School of Public Health,Inner Mongolia Medical University,Hohhot 010110,China)
出处
《科学技术与工程》
北大核心
2018年第32期162-167,共6页
Science Technology and Engineering
基金
国家自然科学基金(81660564)
内蒙古医科大学慢性病分子流行病学创新团队项目(YKD2017KJCXTDO15)
内蒙古自治区2016年度人才开发基金资助
关键词
2型糖尿病
脂联素
基因
单核苷酸多态性
交互作用
type 2 diabetes
adiponectin
gene
single nucleotide polymorphism
interaction
