摘要
【目的】评价柴桂感寒口服液的毒性作用并初步确定其安全范围,为柴桂感寒口服液的兽医临床用药提供参考依据。【方法】选取健康的昆明系小鼠(18.00~22.00 g/只),按1.875、3.750、7.500、15.000和30.000 g/kg的给药剂量设5个处理组,于24 h内分2~3次灌胃给药进行急性毒性试验;另取20只昆明系小鼠,雌雄各半,按60.000 g/kg的剂量灌胃给药进行最大耐受量试验。长期毒性试验则选取健康清洁级SD大鼠80只,随机分为高剂量组(20.000 g/kg)、中剂量组(10.000 g/kg)、低剂量组(5.000 g/kg)和空白对照组(纯化水),每组20只(雌雄各半),给药周期30 d,于停药后第1 d(试验第31 d)和停药后1周(试验第38 d)分别进行眼球采血,用于血常规指标和血清生化指标检测,同时剖检并采集大鼠实质性脏器计算脏器指数及制作石蜡组织切片。【结果】急性毒性试验小鼠灌胃给药后30 min内嗜睡,精神萎靡,但40 min后其生理状态恢复正常,无中毒症状和死亡现象,因而无法计算出半数致死量(LD50)。在最大耐受量试验中,各给药剂量组小鼠也未出现死亡情况,剖检发现其心脏、肝脏、脾脏、肺脏、肾脏、胸腺、胃肠、睾丸(♂)、子宫(♀)和卵巢(♀)等脏器均无任何肉眼可见的特征性病理变化,即小鼠对柴桂感寒口服液的最大耐受量大于60.000 g/kg。长期服用柴桂感寒口服液对大鼠的采食量和体重均无不利影响,不会导致大鼠实质性脏器损伤或发生异常变化,对其血常规指标和血清生化指标也无明显影响;石蜡组织切片观察发现各给药剂量组大鼠肝脏、脾脏和肾脏的组织形态结构与空白对照组一致,均未出现异常病理变化。【结论】柴桂感寒口服液无急性毒性作用,长期连续服用也不会产生毒副作用,兽医临床用药安全性高,可用于治疗风寒感冒等病证。
【Objective】The aim of the present study was to evaluate the toxic effects of Chaigui cold oral liquid and to determine its safety range,and provide reference for veterinary clinical use of Chaigui cold oral liquid.【Method】Twenty healthy Kunming mice(18.00-22.00 g/mouse)were selected for five dose groups in acute toxicity test,and were administered for twice to three times at a dose of 1.875,3.750,7.500,15.000 and 30.000 g/kg in 24 h by intragastric administration.Another 20 Kunming mice in half female and half male were fed with 60.000 g/kg by intragastric administration for the maximum tolerance limit test.The long-term toxicity test selected 80 SD mice of healthy clean grade,randomly divided them into high dose(20.000 g/kg),middle dose(10.000 g/kg),low dose(5.000 g/kg)and blank control(purified water)groups,each group was with 20 mice(half males and half females).The drug administration period was 30 d.On the 1st day after the end of the administration(the 31th day of the test)and one week after the withdrawal(the 38th day of the test),blood was collected from the eye for blood routine index test and serum biochemical indicators test.The mice organs were dissected and collected,the organ index was calculated and the paraffin tissue sections were made.【Result】In acute toxicity test,the mice were lethargic and listless within 30 min after intragastric administration,but all recovered after 40 min without symptoms of poisoning and death.According to the toxicological evaluation criteria,the median lethal dose(LD50)could not be measured.In the maximum tolerance limit test,no death occurred in the mice in each dose group.The heart,liver,spleen,lung,kidney,thymus,intestines and stomach,testis(♂),uterus(♀)and oarium(♀)had no visible pathological changes,which indicated that the maximum tolerance of mice to Chaigui cold oral liquid was greater than 60.000 g/kg.Long-term use of Chaigui cold oral liquid had no adverse effects on the feed intake and body weight of mice,and did not cause abnormal organ da
作者
肖蕊
施君
谢小东
韦秀颖
韦英益
胡庭俊
XIAO Rui;SHI Jun;XIE Xiao-dong;WEI Xiu-ying;WEI Ying-yi;HU Ting-jun(College of Animal Science and Technology,Guangxi University,Nanning 530004,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2018年第11期2327-2334,共8页
Journal of Southern Agriculture
基金
广西创新驱动发展专项项目(桂科AA17204081-2)
南宁市科学研究与技术开发计划项目(20175031-4)
关键词
柴桂感寒口服液
急性毒性
最大耐受量
长期毒性
Chaigui cold oral liquid
acute toxicity
maximum tolerance dose
long-term toxicity