摘要
目的:研究黑果枸杞花色苷对部分睡眠剥夺(PSD)大鼠心肌损伤的保护作用。方法:采用改良多平台睡眠剥夺法,60只SD大鼠随机分为:正常对照组(N组)、PSD模型组(M组)、复方丹参片对照组(CDT组)、PSD+黑果枸杞花色苷低剂量组(LrM-L组),PSD+黑果枸杞花色苷中剂量组(LrM-M组),PSD+黑果枸杞花色苷高剂量组(LrM-H组) 6组,每组10只。6组大鼠均每天灌胃1次,LrM-L、LrM-M、LrM-H分别灌服黑果枸杞花色苷0.25、0.5、1.5 g/(L·kg·d),N、M和CDT组均按体质量灌服等量0.9%NaCl和复方丹参片0.32 g/(L·kg·d)。PSD大鼠每天睡眠剥夺18 h,共持续21d后,分别观察各组大鼠一般情况;取血清和心肌组织检测氧化指标:CK-MB、cTnI、T-AOC、SOD、MDA;并采用ELISA法测定心肌特征性损伤标志物含量; HE染色后观察心肌组织病理学改变。结果:PSD模型组大鼠的心肌损伤最明显,而黑果枸杞花色苷可降低PSD大鼠的心肌损伤,且灌胃剂量与心肌损伤呈量效依赖性;与PSD模型组相比较,黑果枸杞花色苷0.25、0.5、1.5 g/(L·kg·d)组大鼠血清CK-MB、cTnI和心肌MDA水平降低(P<0.05),且随剂量递加而递减;血清T-AOC水平和心肌SOD水平升高(P<0.05),且随剂量增加而增加;上述各指标变化均以1.5 g/(L·kg·d)组尤为明显。结论:黑果枸杞花色苷能有效提高心肌抗过氧化能力,减少睡眠剥夺产生的过量的自由基损伤,从而抑制PSD所致的心肌细胞病理性损害,提示不同给药剂量的黑果枸杞花色苷对部分睡眠剥夺大鼠具有不同程度的心肌保护作用。
Objective:To observe the protective effects of Lycium ruthenicum Murray anthocyanin extracts on the heart injury in rats of partial sleep deprivation(PSD).Methods:Rat model of partial sleep deprivation was induced by the modified multiple platform method(MMPM).Sixty SD rats were equally randomized into six groups,namely:blank control group(group N),partial sleep deprivation group(group M),PSD+low dose Lycium ruthenicum Murray anthocyanin extract(group LrM-L),PSD+middle dose Lycium ruthenicum Murray anthocyanin extract(group LrM-M),and PSD+high dose Lycium ruthenicum Murray anthocyanin extract(group LrM-H).Rats in each group were subjected to intragastric administration once a day.Rats in LrM-L,LrM-M,LrM-H group were given Lycium ruthenicum Murray anthocyanin extract in dose of 0.25,0.5,1.5 g/(L·kg·d),and those in group N,M,and control group were intragastrically treated with equal amount of 0.9%NaCl and compound salvia tablet in dose of 0.32 g/(L·kg·d).Rats were deprived of partial sleep by 18 h per day for consecutive 21 days,and observed on general conditions.Then the serum and myocardial tissue were taken from all rats to determine the oxidative stress index,including creatine kinase isoenzyme(CK-MB),cardiac troponin I(cTnI),total antioxidant capacity(T-AOC),superoxide dismutase(SOD)and malondialdehyde(MDA).ELISA was performed to detect level of characteristic markers of myocardial injury,and the pathological changes of cardiac tissue were examined following H&E staining.Results:Myocardial injury was most notable in rats of PSD.Lycium ruthenicum Murray anthocyanin extract reduced myocardial damage in PSD rats,and the dose of ingastric administration and myocardial injury were in dose-dependent effect.Rats treated with Lycium ruthenicum Murray anthocyanin extract in dose of 0.25,0.5 and 1.5 g/(L·kg·d)had significantly reduced serum CK-MB and cTnI as well as MDA level compared to the PSD models(P<0.05),and the level was reduced with dose increase.Obviously increased level of T-AOC and myocardial SOD was se
作者
温朝玲
刘春红
孙雯雯
汪洋
WEN Chaoling;LIU Chunhong;SUN Wenwen;WANG Yang(Department of Nursing,Anhui College of Traditional Chinese Medicine,Wuhu 241000,China)
出处
《皖南医学院学报》
CAS
2018年第6期523-526,共4页
Journal of Wannan Medical College
基金
安徽省级自然科学重点项目(KJ2017A685)
安徽省高校优秀青年人才支持计划重点项目(gxyqZD2018095)
关键词
黑果枸杞花色苷
部分睡眠剥夺
心肌组织
心肌损伤标志物
抗氧化酶
Lycium ruthenicum Murray anthocyanin extract
partial sleep deprivation
myocardial tissue
marker of myocardial injury
antioxidant enzyme
