摘要
为了揭示厚竹Phyllostachys edulis‘Pachyloen’在孕笋成竹过程中内源激素的分布特征和动态变化,探索内源激素对厚竹孕笋成竹的调控作用,采用酶联免疫吸附法(ELISA),分别测定潜伏芽(鞭芽)、笋芽、冬笋、春笋和新竹中的吲哚-3-乙酸(IAA),赤霉素(GA_3),玉米素核苷(ZR)和脱落酸(ABA)质量分数,并分析差异性。结果表明:厚竹中IAA和ABA质量分数显著高于GA_3和ZR。笋芽膨大期同种内源激素及内源激素总量在潜伏芽、笋芽和冬笋之间差异不显著。竹笋快速生长期,春笋笋箨中的IAA和ZR显著高于潜伏芽,IAA,ABA和ZR显著高于笋肉,笋肉中ABA显著低于潜伏芽。新竹长成后,竹枝和竹叶中内源激素质量分数表现为ABA>IAA>ZR>GA_3,而竹秆和蔸根中则为IAA>ABA>ZR>GA_3。孕笋成竹过程中,不同内源激素及其比值的动态变化不同,其中孕笋过程中IAA,ABA和GA_3,ZR/IAA,ZR/GA_3和GA_3/IAA变化显著,成竹过程中IAA,ABA,ZR/ABA,ZR/IAA,GA_3/IAA和ZR/GA_3变化显著。内源激素的种类、质量分数及相互间的协调或拮抗作用对厚竹的孕笋成竹具有重要的促进或调控功能。
To reveal distribution characteristics and dynamic changes of endogenous hormones in the Phyllostachys edulis‘Pachyloen’bamboo shoot during bamboo shoot development,and to explore the regulation of endogenous hormones on bamboo shoots during development,the enzyme-linked immunosorbent assay(ELISA)test was applied to measure the content of Indole-3-acetic acid(IAA),gibberellin(GA3),zeatin riboside(ZR),and abscisic acid(ABA)in latent buds(rhizome buds),germinating buds,winter shoots,spring shoots,and new bamboo.Analysis included variance analysis and multiple comparisons of a total of 60 samples which were collected in 3 areas and 3 periods.Results showed that the content of IAA and ABA were significantly higher than GA3 and ZR(P<0.05).During the bamboo shoot enlargement period,there were no significant differences(P>0.05)in the four endogenous hormones and the total amount of endogenous hormones in latent buds,shoot buds,and winter shoots.During the rapid shoot growth period,within the sheath of the spring shoot,the contents of IAA and ZR were significantly higher than in latent buds(P<0.05),and the contents of IAA,ABA,and ZR were significantly higher than in shoots(P<0.05).Spring shoots had a significantly lower ABA level than latent buds(P<0.05).After new bamboo growth,endogenous hormones in bamboo leaves and branches were ABA>IAA>ZR>GA3,yet in bamboo stems and roots it was IAA>ABA>ZR>GA3.During development of young bamboo,dynamic changes in content and ratio of endogenous hormones differed with IAA,ABA,GA3,ZR/IAA,ZR/GA3,and GA3/IAA changing significantly during the transition from latent buds to winter shoots(P<0.05);and IAA,ABA,ZR/ABA,ZR/IAA,GA3/IAA,and ZR/GA3 changing significantly during transformation from winter shoots to new bamboo(P<0.05).This research revealed that the type and content of homogenous hormone,as well as antagonistic and synergistic effects among them,were important for promoting or regulating functions in the reproducing process of Ph.edulis‘Pachyloen’.[Ch,2 fig.4 tab.24 ref.]
作者
黎祖尧
张艳华
张雷
李苑
孙娅东
LI Zuyao;LI Zuyao;ZHANG Lei;LI Yuan;SUN Yadong(College of Forestry,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;College of Forestry,Nanjing Forestry University,Nanjing 210037,Jiangsu,China)
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2018年第6期1107-1114,共8页
Journal of Zhejiang A&F University
基金
国家科技支撑计划项目(2015BAD04B01)
江西省重点科技成果转移转化项目(20133ACI90001)
江西省科技支撑计划项目(20121BBF60028)
关键词
植物学
厚竹
内源激素
分布
动态变化
botany
Phyllostachys edulis‘Pachyloen’
endogenous hormone
distribution
dynamic change
