摘要
本试验旨在研究假药提取物对断奶仔猪回肠黏膜屏障的影响。将80头21日龄断奶仔猪随机分为4组,分别在基础日粮中添加0、50、100、200 mg/kg假药提取物,饲喂28 d。分别测定仔猪的生长性能、血清生化指标以及回肠组织形态、通透性和回肠黏膜中相关连接蛋白的mRNA表达。结果表明:日粮中添加50 mg/kg假药提取物可显著提高断奶仔猪平均日采食量和日增重(P<0.05);假药提取物能显著降低仔猪血清中D-乳酸、内毒素以及二胺氧化酶浓度(P<0.05),改善回肠黏膜形态;显著提高了仔猪回肠黏膜中咬合蛋白、封闭蛋白-1和钠氢离子交换蛋白-3 mRNA表达(P<0.05)。上述结果表明,日粮中添加假药提取物能改善仔猪回肠黏膜形态、降低肠道通透性、上调紧密连接蛋白和钠氢离子交换蛋白的mRNA表达,从而保护断奶仔猪肠道黏膜屏障功能。
The study was conducted to investigate the effects of Piper sarmentosum extracts(PSE)on intestinal morphology and intestinal permeability of weaned piglets.A total of eighty 21-d-old weaned piglets were selected and randomly allocated into four treatment groups with five replicates of 4 pigs each.Pigs were fed a basal diet supplemented with PSE at 0,50,100 and 200 mg/kg for 28 days.The results show that the diet supplemented with PSE at 50 mg/kg increased the average daily gain and average daily feed intake of piglets(P<0.05).Meanwhile,diet PSE supplementation reduced the levels of DAO,D-LA and ET-1 in serum of piglets(P<0.05),and improved ileum morphology of weaned piglets.Compared with the control group,the mRNA expression of Occludin,Claudin-1 and NHE-3 in ileum of weaned piglets fed diets with PSE increased significantly(P<0.05).The conclusion showed that diet supplementation with PSE could improve ileum morphology and tight junction protein expression,and decrease intestinal permeability,and then protect intestinal mucosal barrier function in weaned piglets.
作者
王定发
周璐丽
胡海超
周汉林
WANG Ding-fa;ZHOU Lu-li;HU Hai-chao;ZHOU Han-lin(Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Hainan Danzhou,571737,China)
出处
《中国畜牧杂志》
CAS
北大核心
2018年第11期110-114,共5页
Chinese Journal of Animal Science
基金
中国热带农业科学院基本科研业务费专项资金(1630032017036)
海南省自然科学基金(317266)
关键词
假蒟提取物
断奶仔猪
肠道黏膜
通透性
Piper sarmentosum extracts
Weaned piglets
Intestinal mucosa
Intestinal permeability
