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葛根素对脑缺血再灌注大鼠神经功能与大脑皮质突触形态结构及参数的影响 被引量:14

Effects of puerarin on neurological function,and synaptic morphological structure and parameter of cerebral cortex in cerebral ischemia-reperfusion rats
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摘要 目的:观察缺血再灌注(IR)大脑皮质突触形态结构及参数的变化,探讨葛根素(Pue)干预的神经保护作用。方法:将69只SD大鼠随机分为假手术组(sham组)、模型组(IR组)、Pue组和尼莫地平(NIM)阳性对照组(NIM组),sham组15只,后3组每组18只。采用线栓法制作局灶性大脑中动脉IR模型,缺血2 h再灌注24 h后,Pue组和NIM组分别注射Pue 8 mg·kg^(-1)·d^(-1)和NIM 1 mg·kg^(-1)·d^(-1),sham组和IR组给予等体积生理盐水。于用药后3、7和14 d进行改良神经功能缺损评分,而后取缺血侧大脑皮质,电镜观察突触超微结构变化,测量分析突触界面曲率、突触间隙宽度、突触后致密物厚度等参数。结果:与IR组相比,相应时点Pue组和NIM组神经功能缺损评分均降低(P<0.05),这2组的差异无统计学意义。电镜下观察IR组突触前后膜和突触间隙模糊,突触前成分内突触囊泡减少,突触后致密带密度降低;Pue组和NIM组较IR组突触密集,凹形突触增加,常见2个活性点,突触后致密物增厚,间隙清晰,患侧皮质半暗带突触界面曲率增加,突触后致密物增厚,突触间隙减小(P<0.05);7 d组和14 d组的突触各形态结构参数均优于3 d组(P<0.05),两者之间的差异无统计学显著性。结论:Pue有可能通过修复突触结构,促进脑IR大鼠神经功能恢复。 AIM:To investigate the protective effect of puerarin(Pue)on the neural function in a rat model of cerebral ischemia-reperfusion(IR)injury by observing synaptic morphological structural alterations and the parameter variations in brain cortex.METHODS:Male SD rats(n=69)were randomly divided into sham operation group(sham group,n=15),model group(IR group,n=18),Pue group(n=18)and nimodipine(NIM)positive control group(NIM group,n=18).The model of cerebral IR injury was established by a suture method.After ischemia for 2 h and reperfusion for 24 h,the rats in Pue group and NIM group were injected with Pue at 8 mg·kg-1·d-1 and NIM at 1 mg·kg-1·d-1,respectively,while the rats in sham group and IR group were injected with the same volume of saline.The modified neurological severity scores were evaluated at 3,7 and 14 d,the ultrastructural changes of the synapses at the ischemic side of cerebral cortex were observed under transmission electron microscope,and the postsynaptic density was analyzed by Quantiment-520 image analysis software.RESULTS:Compared with IR group,the scores of neurological deficit were both reduced in Pue group and NIM group(P<0.05),and the difference between Pue group and NIM group was not obvious.The presynaptic and postsynaptic membranes and synaptic cleft were indistinct under electron microscope in IR group,the number of synaptic vesicles in presynaptic component was reduced,and the density of postsynaptic dense zone was decreased.The synapses were dense in Pue group and NIM group compared with IR group,the dented synapses were increased,2 active synapses were often presented,postsynaptic density was incrassated and the synaptic cleft was clear.The curvature of synaptic interface was increased,the postsynaptic density was thickened,and the synaptic cleft was narrow in ischemic penumbra(P<0.05).The parameters of synaptic morphological structure at 7 d and 14 d were better than those at 3 d(P<0.05).CONCLUSION:The treatment with Pue promotes neural functional recovery against cerebral IR injury i
作者 李晓伟 王跃 韩硕 孙永奇 李玲玲 刘壮 张晓晟 刘月 赵淑敏 纪海茹 LI Xiao-wei;WANG Yue;HAN Shuo;SUN Yong-qi;LI Ling-ling;LIU Zhuang;ZHANG Xiao-sheng;LIU Yue;ZHAO Shu-min;JI Hai-ru(Chengde Medical University,Chengde 067000,China)
机构地区 承德医学院
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2018年第11期2043-2047,共5页 Chinese Journal of Pathophysiology
基金 国家级大学生创新创业训练计划项目(No.201610093004)
关键词 葛根素 缺血再灌注损伤 突触 突触后致密物 Puerarin Ischemia-reperfusion injury Synapse Postsynaptic density
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