摘要
目的检测并分析肝细胞癌患者血清异常凝血酶原Ⅱ(PIVKAⅡ)、甲胎蛋白(AFP)与血清铁蛋白(Ferrtin)的表达水平,探讨以上指标单独及联合检测对肝细胞癌的诊断价值。方法用化学发光法检测40例肝细胞癌,37例肝硬化和39例慢性肝炎患者(阴性对照),42例体检健康者(健康对照)血清中PIVKAⅡ、AFP、Ferrtin的表达,分析癌症患者与其各病理特征的关系,采用Pearson线性相关分析各项目的相关性,并采用ROC曲线分析指标单独与联合检测肝细胞癌的诊断效能。结果肝细胞癌组PIVKAⅡ、AFP、Ferrtin水平均明显高于阴性对照组及健康对照组,差异均有统计学意义(P<0.05)。癌症患者病理分期越晚,分化程度越低,PIVKAⅡ、AFP水平越高;有腹水组AFP水平明显高于无腹水组,淋巴结转移组的PIVKAⅡ水平明显高于无转移组(均P<0.05)。Pearson线性相关分析显示,PIVKAⅡ、AFP、Ferrtin 3个指标彼此之间呈正相关关系(均P<0.05);3个指标联合检测ROC曲线下面积、灵敏度及特异度均大于各个指标单独检测(P<0.05)。结论联合检测PIVKAⅡ、AFP、Ferrtin比单独检测对肝细胞癌有更好的诊断价值,对肝细胞癌的诊断及鉴别诊断有重要意义。
Objective To detect and analyze the levels of abnormal prothrombinⅡ(PIVKAⅡ),alpha-fetoprotein(AFP)and serum ferritin(Ferrtin)in patients with hepatocellular carcinoma,and to explore the diagnostic value of the above indices alone and in combination for hepatocellular carcinoma.Methods The expressions of PIVKAⅡ,AFP and Ferrtin in serum of 40 cases of hepatocellular carcinoma,37 cases of cirrhosis and 39 cases of chronic hepatitis(negative control)and 42 cases of healthy controls were detected by chemiluminescence.The relationship between cancer patients and their pathological features was analyzed,and the correlation of each item was analyzed by Pearson analysis method.The diagnostic efficacy of hepatocellular carcinoma was assessed by ROC curve analysis individually and jointly.Results The levels of PIVKAⅡ,AFP and Ferrtin in the hepatocellular carcinoma group were significantly higher than those in the negative control group and the healthy control group(P<0.05).The later the stage of cancer,the lower the degree of differentiation,the higher the level of PIVKAⅡ,AFP.AFP level in ascites group was significantly higher than that in non-ascites group,and PIVKAⅡlevel in lymph node metastasis group was significantly higher than that in non-metastasis group(all P<0.05).Pearson analysis showed that there was a positive correlation among the three indexes(all P<0.05),and the area under the ROC curve,sensitivity and specificity of the three indexes were greater than those of the individual indexes(P<0.05).Conclusion Combined detection of PIVKAⅡ,AFP and Ferrtin has better diagnostic value for hepatocellular carcinoma than single detection.It is important for the diagnosis and differential diagnosis of hepatocellular carcinoma.
作者
赵芬
郭庆波
曲凯
梁欢
ZHAO Fen;GUO Qingbo;QU Kai;LIANG Huan(Department of Clinical Laboratory,Fuhsan District People′s Hospital,Yantai,Shandong 264000,China;Department of Clinical Laboratory,Weifang Yidu Central Hospital,Weifang,Shandong 262500,China;Department of Hepatobiliary Surgery,the First Affiliated Hospital of Xi′an Jiaotong University,Xi′an,Shaanxi 710061,China;Department of Emergency,the First Affiliated Hospital of Xi′an Jiaotong University,Xi′an,Shaanxi 710061,China)
出处
《国际检验医学杂志》
CAS
2018年第21期2615-2617,2621,共4页
International Journal of Laboratory Medicine
关键词
异常凝血酶原Ⅱ
甲胎蛋白
铁蛋白
肝细胞癌
abnormal prothrombinⅡ
alpha-fetoprotein
ferritin
hepatocellular carcinoma
