摘要
目的在分析湖北省两县市的腮腺炎疾病流行情况基础上,对腮腺炎病毒血清学抗体检测方法进行比较分析。方法收集2010～2016年当阳市和谷城县流行性腮腺炎疫情资料进行疾病流行趋势描述。同时依托于湖北省当阳市和谷城县开展的腮腺炎减毒活疫苗(人二倍体细胞)的随机、双盲、阳性疫苗对照的Ⅱ期临床试验,分别采集491例当阳市及526例谷城县8～24个月龄受试者的免疫前血液样品进行腮腺炎病毒抗体水平检测。检测方法包括中和抗体检测法和血凝抑制抗体检测法,每种方法又分别以F基因型或A基因型毒株作为指示病毒或血凝素;就4种检测结果对两地腮腺炎抗体阳性率进行组间比较,并使用同一检测方法对不同基因型毒株的检测结果进行比较分析。结果当阳市2012～2013年达到腮腺炎发病高峰,而谷城县的发病高峰年则在2014～2015年。血清学检测结果表明,A基因型中和抗体法、A基因型血凝抑制抗体法及F基因型血凝抑制抗体法在当阳市、谷城县两地的抗体阳性率比较,差异均无统计学意义;而当使用F基因型中和抗体检测法时,谷城腮腺炎抗体阳性率为7. 0%,当阳市为0. 4%,两地抗体阳性率比较差异有统计学意义(P=0. 000)。使用血凝抑制抗体法同时检测两种基因型毒株的检测结果差异无统计学意义;而运用中和抗体法同时检测则发现,谷城县抗F基因型病毒抗体水平呈阳性而抗A基因型病毒抗体水平呈阴性的比例高达6. 6%,抗A基因型病毒抗体水平呈阳性而抗F基因型病毒抗体水平呈阴性比例则为0. 0%,两种基因型毒株的中和抗体检测结果比较差异有统计学意义(P=0. 000)。结论采用F基因型毒株作为指示病毒,同时使用中和抗体检测方法,能提高检测结果的敏感度,从而有效评价腮腺炎血清学抗体水平。
Objective Based on the prevalence of mumps in the two regions of Hubei Province,the methods of antibody testing for mumps were analysed.Methods The epidemiological data of mumps in Dangyang City and Gucheng County from 2010 to 2016 were collected to describe the prevalence of mumps.Simultaneously,on the basis of the randomized,double blind,positive-controlled phaseⅡclinical trial conducted in Dangyang and Gucheng counties,Hubei Province,491 individuals aged 8-24 months in Dangyang City and 526 individuals aged 8-24 months in Gucheng County were collected for testing of the mumps antibody and the test methods include neutralization test and hemagglutination inhibition test,each of which takes F genotype and A genotype strains as the indicator virus or hemagglutinin,respectively.Then the paper compares the positive rates of mumps antibody between the two regions and analyzes the test results of two genotypes by using the same antibody testing method.Results Mumps reached the peak in Dangyang city in the year 2012-2013,while the peak year in Gucheng County was year 2014-2015.Antibody testing results showed that there is no statistically significance in the positive rates of mumps antibody between Dangyang and Gucheng regions by using the neutralizing test with A genotype method,hemagglutination inhibition test with A genotype method and hemagglutination inhibition test with F genotype method.When the neutralizing test with A genotype method was used,the positive rate of mumps antibody in Gucheng was 7%,while 0.4%in Dangyang,there is statistically significance in the positive rates of mumps antibody between the two regions(P=0.000).There was no significant difference in the results between two genotypes by using the hemagglutination inhibition test.The inconsistency rate that anti-F genotype virus antibody level displays positive while anti-A genotype virus antibody level displays negative was 6.6%,and the inconsistency rate that anti-A genotype virus antibody level displays positive while anti-F genotype virus ant
作者
殷琼洲
夏龙辉
李菁
李琦涵
李红
崔晓雨
任群慧
何永华
廖芸
蒋国润
赵志梅
王兆
李琼
梁燕
杨北方
Yin Qiongzhou;Xia Longhui;Li Jing(Institute of Medical Biology,Chinese Academy of Medical Sciences,Peking Union Medical College,Yunnan 650118,China)
出处
《医学研究杂志》
2018年第10期49-53,40,共6页
Journal of Medical Research
基金
中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-019)
云南省重大科技专项基金资助项目(2015ZJ001)
