摘要
目的观察布加综合征(BCS)患者血清中基质金属蛋白酶抑制因子-1(TIMP-1)、核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)的水平变化,并探讨其意义。方法选取BCS患者103例(BCS组),肝硬化患者55例(对照肝硬化组),体检中心健康人群130例(正常对照组)为调查对象。采用ELISA法检测三组血清中TIMP-1、NF-κB、TNF-α水平,并采用Spearman对其进行相关性分析。结果 BCS组和对照肝硬化组血清TIMP-1、NF-κB、TNF-α水平均高于正常对照组(P均<0. 05),BCS组血清TIMP-1水平低于对照肝硬化组(P <0. 05),NF-κB、TNF-α水平高于对照肝硬化组(P均<0. 05); BCS合并肝硬化者和BCS合并肝癌者血清TIMP-1、NF-κB、TNF-α水平均高于单纯BCS者(P均<0. 05),BCS合并肝癌者血清TIMP-1、NF-κB、TNF-α水平高于BCS合并淤血性肝硬化者(P均<0. 05); BCS组血清NF-κB和TIMP-1呈正相关(r=0. 659,P <0. 001),TNF-α和TIMP-1呈正相关(r=0. 774,P <0. 001),NF-κB和TNF-α呈正相关(r=0. 601,P <0. 001),血清TIMP-1、NF-κB、TNF-α与BCS疾病严重程度均呈正相关(r分别为0. 628、0. 742、0. 621,P均<0. 001); ROC曲线分析结果显示,血清TIMP-1、NF-κB、TNF-α诊断BCS合并肝硬化的曲线下面积分别为0. 791、0. 901、0. 808。结论随着BCS病程进展,血清TIMP-1、NF-κB、TNF-α水平逐渐升高; TIMP-1、NF-κB、TNF-α可作为判断BCS患者是否发生肝硬化的参考指标。
Objective To observe the changes of serum levels of tissue inhibitor of matrix-1(TIMP-1),nuclear factor-κB(NF-κB)and tumor necrosis factor-α(TNF-α)in patients with Budd-Chiari syndrome(BCS),and to explore its significance.Methods One hundred and three patients with BCS(BCS group),55 patients with cirrhosis(control group),and 130 healthy people(normal control group)in the medical examination center were selected.The levels of serum TIMP-1,NF-κB and TNF-αin the three groups were tested by ELISA,and the correlation was analyzed by Spearman.Results The levels of serum TIMP-1,NF-κB,and TNF-αin the BCS group and control group were all higher than those in the normal control group(all P<0.05);serum TIMP-1 level in the BCS group was lower,and NF-κB and TNF-αlevels were higher than those in the control group(all P<0.05);the levels of serum TIMP-1,NF-κB and TNF-αin patients with BCS and cirrhosis and patients with BCS and liver cancer were all higher than those in pure BCS patients(all P<0.05),and the levels of three indexes in patients with BCS and liver cancer were higher than those in patients with BCS and cirrhosis(all P<0.05).In the BCS group,NF-κB was positively correlated with TIMP-1(r=0.659,P<0.001),TNF-αwas positively correlated with TIMP-1(r=0.774,P<0.001),and TNF-αwas positively correlated with NF-κB(r=0.601,P<0.01);TIMP-1,NF-κB and TNF-αhad a positive correlation with the severity of BCS disease(r=0.628,0.742,and 0.621,respectively;P<0.001).The ROC curve analysis showed that the sub-curve scores of serum TIMP-1,NF-κB and TNF-a in the diagnosis of BCS and cirrhosis were 0.791,0.901,and 0.808,respectively.Conclusion With the progression of BCS,the levels of serum TIMP-1,NF-κB,and TNF-αincrease gradually,and TIMP-1,NF-κB,or TNF-αcan be used as the reference indexes to determine whether BCS patients have cirrhosis or not.
作者
卢慧敏
杨丹丹
张端强
黄琦
高修银
LU Huimin;YANG Dandan;ZHANG Ruiqiang;HUANG Qi;GAO Xiuyin(School of Public Health,Xuzhou Medical University,Xuzhou 221000,China)
出处
《山东医药》
CAS
2018年第41期10-13,共4页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81172604)
徐州市科技计划项目(KC15SX002)
