摘要
目的探讨羊骨髓肽(sheep bone marrow peptides,MP)及其钙螯合物(MP-Ca)对诱导分化的OC形成和活性的影响。方法将MP与CaCl_2螯合制备MP-Ca,以RAW264.7为破骨前体细胞模型,建立OC的RANKL诱导体系,以MP和MP-Ca干预诱导过程,确定二者在最适作用浓度下对诱导曲线、TRAP活性和骨吸收陷窝数的影响。结果诱导后第5天,细胞呈现OC典型形态,TRAP染色呈阳性并在共培养的骨片上形成骨吸收陷窝,MTT检测时OD_(490)达到峰值;以第5天作为检测时间,MP与MP-Ca均以10~3μg/mL时OD_(490)最低;以10~3μg/mL MP和MP-Ca干预诱导体系,除第1天外,干预组OD_(490)均显著低于空白组(P<0.05);在第5天,MP-Ca组的OD_(490)和TRAP活性均显著低于MP组(P<0.05);各干预组的骨吸收陷窝数均显著低于空白组,且MP组显著低于MP-Ca组(P<0.05);17β-雌二醇(10μg/mL)的各检测指标均显著低于干预组(P<0.05)。结论虽效果不及雌激素,MP和MP-Ca均可抑制OC的诱导形成及其破骨活性,MP-Ca对OC诱导形成的抑制作用强于MP。
Objective Osteoclast numbers and activity are directly related to bone turnover and metabolism.This study aimed to investigate the effects of sheep bone marrow peptides(MP)and calcium-chelated MP(MP-Ca)on the formation and activity of RANKL-induced osteoclasts.Methods MP-Ca was prepared by a chelating reaction between MPs and CaCl 2.RAW264.7 cells were induced to osteoclasts by RANKL treatment,and cell proliferation was examined using the MTT assay.Induction was intervened by MP and MP-Ca to determine optimal concentrations and to investigate their effects on induction curves,TRAP activity,and the number of lacunae formed in bone slices.Results Under the induction system,on day 5,cultured cells were took on typical morphology of mature osteoclasts,TRAP staining was positive,resorptive pits formed,and the peak OD 490 value was achieved.The lowest OD 490 value was achieved when 10 3μg/mL MP or MP-Ca were added into the induction system on day 5.When 10 3μg/mL MP or MP-Ca was added to the induction system,except on day 1,OD 490 values from days 2-8 were significantly lower than the blank group(P<0.05).In addition,the OD 490 value and TRAP activity of the MP-Ca group were markedly lower than the MP group(P<0.05).Pit numbers in the intervention groups were notably lower than the blank group,and the number of lacunae in the MP group was significantly lower than the MP-Ca group(P<0.05).All indices checked in the 17-β-estradiol group were lower than the intervention groups(P<0.05).Conclusions Although inferior to estrogen,both MP and MP-Ca inhibite the RANKL-induced formation and the resorptive activity of osteoclasts.Moreover,MP-Ca was more effective at inhibiting osteoclast formation than MP.
作者
陈静
姚薇
韩克光
郑明学
古少鹏
张鼎
田文霞
霍乃蕊
CHEN Jing;YAO Wei;HAN Keguang;ZHENG Mingxue;GU Shaopeng;ZHANG Ding;TIAN Wenxia;HUO Nairui(College of Animal Science And Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China;College of Food And Engineering,Shanxi Agricultural University,Taigu 030801)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2018年第5期574-579,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金(31201347)
山西省回国留学人员项目(2014-042)~~
关键词
破骨细胞
骨髓肽
肽钙螯合物
17Β-雌二醇
骨代谢
osteoclasts
bone marrow peptides
calcium-chelated peptides
17-β-estradiol
bone metabolism of interest statement.
