摘要
采用多种色谱法从思茅山橙根部分的生物碱粗提物分离得到10个生物碱类化合物,利用波谱等方法对其结构进行鉴定,包括3种不同的生物碱骨架结构,分别为喹啉型生物碱:melodinhenine D(1)、melaxilline A(2)、11-methoxytabersonine(3)、meloscandonine(4)、melodinine T(5);白坚木型生物碱:venalstonidine(6)、vandrikidine(7);长春曼胺型生物碱:14,15-dehydrovincine (8)、14,15-dehydro-16-epi-vincamine (9)、△14-vincanol(10)。除化合物1,5和8外,其余化合物均首次从思茅山橙中分离得到。采用MTT法对分离所得的化合物进行体外抗人乳腺癌细胞MCF-7活性测试,结果显示化合物3表现出显著的细胞毒活性,其IC_(50)值为23. 4μM。
Ten alkaloids were isolated from the roots of Melodinus henryi by a combination of various chromatographic techniques,including column chromatography over silica gel,pH-zone-refining high-speed counter-current chromatography,and reversed-phase HPLC,etc.The structures of these alkaloids were determined on the basis of spectroscopic analysis including MS and NMR data,which were divided into three types,including quinine-type alkaloids:melodinhenine D(1),melaxilline A(2),11-methoxytabersonine(3),meloscandonine(4),melodinine T(5);aspidospermidine-type alkaloids:venalstonidine(6),vandrikidine(7);vincadine-type alkaloids:14,15-dehydrovincine(8),14,15-dehydro-16-epi-vincamine(9),△14-vincanol(10).All the compounds except 1,5 and 8 were isolated from Melodinus henryi for the first time.All the compounds were tested for their cytotoxicities against human breast cancer MCF-7 cells using the MTT method,and compound 3 exhibited significant cytotoxic effect with IC 50 value of 23.4μM.
作者
于金倩
孙晓炜
闫慧娇
方磊
王晓
YU Jin-qian;SUN Xiao-wei;YAN Hui-jiao;FANG Lei;WANG Xiao(Shandong Key Laboratory of TCM Quality Control Technology,Shandong Analysis and Test Center, Qilu University Of Technology(Shandong Academy of Sciences);School of Chemistry and Pharmaceutical Engineering,Qilu University of Technology,Jinan,250014,Shandong;College of Biological Sciences and Technology,University of Jinan,Jinan 250022,Shandong)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2018年第10期1738-1742,共5页
Natural Product Research and Development
基金
山东省自然科学基金(ZR2016YL006)
山东省自然科学基金面上项目(ZR2017MH019)
