摘要
利用病原微生物革兰氏阳性藤黄微球菌(Micrococcus luteus)和革兰氏阴性鳗弧菌(Vibrio anguillarum)分别侵染活体青蛤,采用第二代测序Mi Seq技术测序,构建了青蛤血淋巴中应答2种病原物的转录组文库。根据KEGG等数据库注释信息对Unigene进行生物学通路注释,预测出青蛤免疫信号通路的差异性表达基因及相关注释信息。结果发现,共注释并筛选出2 605个差异表达基因,其中893个基因注释到15个免疫相关的通路中。该研究可为探索青蛤的免疫识别方式和信号传导路径提供依据。
Cyclina sinensis were injected with Micrococcus luteus and Vibrio anguillarum respectively.Using the Illumina Miseq system of second-generation sequencing technology,two transcriptome library of hemolymph was constructed.Unigene could be annotated to biological pathway by KEGG database to predict differential expression network of immune signaling pathway in C.sinensis.The results showed that 2 605 differentially expressed genes were found and a total of 893 genes annotated to 15 immune related pathways.This research could provide basis for exploring immune recognition and signal transduction pathway in C.sinensis.
作者
赵婷
潘宝平
ZHAO Ting;PAN Bao-ping(Tianjin Medical College,Tianjin 300222;Tianjin Key Laboratory of Animal and Plant Resistance,College of Life Sciences,Tianjin Normal University,Tianjin 300387)
出处
《安徽农业科学》
CAS
2018年第27期84-87,共4页
Journal of Anhui Agricultural Sciences
基金
天津市科委应用基础与前沿技术重点项目(12JCZDJC22800)
关键词
青蛤
转录组文库
鳗弧菌
藤黄微球菌
免疫相关基因
Cyclina sinensis
Transcriptome library
Vibrio anguillarum
Micrococcus luteus
Immune-related genes
