摘要
【目的】为探究CO_2增施对弱光区设施黄瓜叶片光系统II、光系统I、跨膜质子梯度及产量的影响。【方法】以‘川翠3号’黄瓜为材料,利用吊袋式CO_2气肥进行黄瓜的CO_2增施处理,采用分光光度计、LI-6400便携式光合测定仪和Dual-PAM-100双通道荧光测定仪分别测定处理和对照黄瓜叶片的光合色素含量、叶片气体交换参数、光系统II和光系统I荧光参数及跨膜质子梯度的550~515 nm差示信号等参数;此外,测定黄瓜叶片的叶面积、单果重、单株结果数、株高及产量等指标。【结果】CO_2增施使黄瓜叶片叶面积、叶绿素b、总叶绿素含量、表观量子效率(AQE)增高,而叶绿素a/b下降;PSII和PSI的最大电子传递速率和跨膜电位(Δψ)增高导致pmf增高。此外,CO_2增施使得黄瓜单果重、单株结果数及株高均有增加,小区产量比对照增加73.8%。由此可见,叶片接受的光能更多的用于CO_2同化作用,同时提高了黄瓜叶片的弱光利用率和抗光氧化的能力,最终通过增加单果重和单株结果数导致其产量的增加。【结论】本研究结果不仅进一步揭示了弱光区黄瓜光合系统对CO_2浓度升高的响应机制,还为四川弱光地区春季黄瓜促成栽培中应用吊袋式CO_2施肥方式提供了理论依据。
【Objective】In this paper,the effect of CO 2 enrichment on the photosystem II(PS II),photosystem I(PS I)and proton motive force in cucumber leaves and its yield were investigated in poor light region by using hanging bag type CO 2 fertilizer.【Method】The chlorophyll content,gas exchange parameters,photosystem II and photosystem I fluorescence parameters of the treated and controlled cucumber leaves were measured by spectrophotometer,LI-6400 portable photosynthesis measuring instrument and Dual-PAM-100 fluorometer.In addition,the leaf area,fruit weight,fruit number per plant,plant height and yield of cucumber leaves were measured.【Result】The results showed that elevated CO 2 concentration increased the cucumber leaf area,chlorophyll b content,total chlorophyll content and apparent quantum efficiency(AQE),decreased the chlorophyll a/b.Elevated CO 2 concentration increased maxium electric transportation rate of PSII and PSI,membrane potential(Δψ)and proton motive force(pmf).Elevated CO 2 concentration increased fruit weight,fruit number per plant.The plot yield of cucumber increased 73.8%compared to no CO 2 enrichment(CK).The results indicated that the light received by the leaves is more used for CO 2 assimilation,and the weak light utilization and anti-photooxidation of cucumber leaves are improved under CO 2 enrichment condition.Finally,the yield of fruit is increased by increasing fruit weight and fruit number per plant.【Conclusion】The results of this study not only further reveal the response mechanism of cucumber photosynthetic system to the increase of CO 2 concentration,but also provide a theoretical basis for the application of hanging bag type CO 2 fertilizer in spring cucumber cultivation in poor light region of Sichuan.
作者
张泽锦
唐丽
李跃建
刘小俊
ZHANG Ze-jin;TANG Li;LI Yue-jian;LIU Xiao-jun(Horticulture Research Institute,Sichuan Academy of Agricultural Sciences/Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province,Sichuan Chengdu 610066,China;Key Laboratory of Horticultural Crop Biology and Germplasm Enhancement in Southwest,Ministry of Agriculture,Sichuan Chengdu 610066,China)
出处
《西南农业学报》
CSCD
北大核心
2018年第8期1599-1605,共7页
Southwest China Journal of Agricultural Sciences
基金
四川省财政创新能力提升工程"CO2增施在四川地区设施作物生产中的应用"(2015JSCX-016)
四川省财政专项"四川弱光区设施蔬菜栽培技术研究与示范"(2016YTS-001)
关键词
CO2升高
PSII
PSI
跨膜质子梯度
黄瓜
Elevated CO 2 concentration
Photosystem II
Photosystem I
Proton motive force
Cucumber
