摘要
目的对五指山小型猪PPARγ2基因启动子调控区域多态性进行分析。方法根据猪PPARγ2启动子调控区域2200 bp设计5对引物,对57头封闭群五指山小型猪的多态性进行检测,并用多种生物信息学软件对调控区域的启动子、转录因子结合位点、RNA二级结构、Cp G岛进行预测分析。结果筛选到9个单核苷酸多态性(single nucleotide polymorphisms,SNPs)位点,分别是:-1595T/C、-1534G/A、-1262C/A、-1220C/A、-1017A/G、-963A/G、-955G/A、-866A/G、-333G/A。SNPs都没有处在软件识别的启动子区域中,但-333G/A突变位于核心启动子(-656^-23 bp)区域。突变的SNP位点附近转录因子结合位点会发生改变,突变前后RNA二级结构最小自由能发生变化,其结构也发生显著变化。目标序列未找到Cp G岛。结论筛选到的五指山小型猪PPARγ2启动子调控区域SNPs可能对该基因的表达调控产生影响,为进一步研究其功能奠定基础。
Objective To screen the polymorphisms of the promoter regulatory region of the PPARγ2 gene in Wuzhishan minipigs.Methods Five pairs of primers were designed based on the promoter regulatory region of the PPARγ2 gene in pigs.Then,the polymorphisms of 57 outbred Wuzhishan minipigs were detected;moreover,the promoters,transcription factor?binding sites,and secondary structures of RNA and CpG islands of the regulatory region were predicted and analyzed by various types of bioinformatic software.Results Nine single?nucleotide polymorphisms(SNPs)were found,which were-1595T/C,-1534G/A,-1262C/A,-1220C/A,-1017A/G,-963A/G,-955G/A,-866A/G,and-333G/A.SNPs were not located in the region of promoters that were identified by the software,but the-333G/A mutation was located in the core promoter region(-656 to-23 bp).Transcription factor?binding sites were altered near the mutational locus.The minimum free energy of secondary structures of RNA was changed and the structure of RNA was significantly altered.CpG islands were not found in the target sequence.Conclusions The screened SNPs of the promoter region of the PPARγ2 gene in Wuzhishan minipigs may affect regulation of the expression of this gene,which lays the foundation for further study of its function.
作者
潘金春
闵凡贵
王希龙
PAN Jinchun;MIN Fangui;WANG Xilong(Guangdong Laboratory Animals Monitoring Institute,Guangzhou 510663,China;Guangdong Provincial Key Laboratory of Laboratory Animals,Guangzhou 510663)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第9期21-26,共6页
Chinese Journal of Comparative Medicine
基金
广东省科技计划项目(编号:2017B030314171
2017A070702001)
广州市科技计划项目(编号:201707010376)
