摘要
背景:建立简便可行、失败率低的激素性股骨头缺血性坏死动物模型,是研究激素性股骨头缺血性坏死的主要途径。目的:建立激素性股骨头缺血性坏死动物模型,并进行病理学、影像学、骨/血清生化、血流变学评价。方法:将20只大耳白兔随机分2组,实验组经耳缘静脉注射马血清10mL/kg,14d后再注射1次,再次注射14 d后,臀肌注射醋酸泼尼松龙8 mg/kg,1次/d,连续注射6 d,建立激素性股骨头缺血性坏死模型;对照组注射等量生理盐水。造模后8周,进行股骨头X射线、MRI检查;造模后10周,进行骨生化、血清生化、血液流变学及股骨头组织学观察。结果与结论:(1)X射线:对照组骨皮质较厚,骨小梁清晰;实验组关节间隙变窄,骨皮质显著变薄,骨密度明显降低,骨小梁重度紊乱;(2)MRI检测:对照组未见股骨头坏死现象,实验组表现典型的双线征;(3)骨生化:实验组氨基己糖、钙/羟脯胺酸、氨基己糖/羟脯胺酸、钙含量低于对照组(P <0.05);(4)血清生化:实验组钙磷乘积、胆固醇、钙水平低于对照组(P <0.05),三酰甘油水平高于对照组(P <0.05);(5)血液流变学:实验组全血高切黏度及低切黏度、血浆黏度比及红细胞压积高于对照组(P<0.05);(6)组织学观察:实验组骨小梁中大部分骨细胞坏死,未坏死的骨细胞肿胀,细胞核严重变形、边聚,还有细胞核碎化、溶解、空鼓等现象,多核破骨细胞明显增多,造血干细胞明显减少;对照组未见上述现象;(7)结果表明,采用马血清联合大剂量和醋酸泼尼松龙可得到较理想的激素性兔股骨头缺血性坏死动物模型,X射线、MRI检查及骨/血清生化、血流变学评价可作为激素性股骨头缺血性坏死辅助诊断手段。
BACKGROUND:Establishing a simple,feasible and high-efficient animal model of steroid-induced avascular necrosis of the femoral head(SANFH)is the main method to study SANFH.OBJECTIVE:To establish the animal models of SANFH,and to investigate the pathology,imaging,bone/serum biochemistry and hemorheology.METHODS:Twenty rabbits were randomly divided into two groups.The rabbits were subjected to injection of 10 mL/kg horse serum via the ear vein twice,with 14 days in between;at 14 days after the final venous injection,the gluteal muscle was injected with 8 mg/kg prednisolone acetate,once daily for 6 consecutive days,so as to establish the model of SANFH(experimental group).The remaining rabbits were injected with the same volume of normal saline(control group).At 8 weeks after modeling,X-ray and MRI examinations of the femoral head were performed.At 10 weeks after modeling,the bone/serum biochemistry,hemorheology,and histological observation of the femoral head were conducted.RESULTS AND CONCLUSION:X-ray:in the control group,the cortical bone was thick and the trabecular bone was clear.In the experimental group,the joint space became narrow,the cortical bone became thinner,the bone density decreased significantly,and the trabecular bone was severely in disorder.MRI examination showed that there was no necrotic femoral head in the control group,and the typical bilinear sign was observed in the experimental group.Bone biochemistry:the contents of hexosamine,calcium/hydroxyproline,hexose/hydroxyproline,and calcium in the experimental group were significantly lower than those in the control group(P<0.05).Serum biochemistry:the calcium-phosphorus product,cholesterol,and calcium levels in the experimental group were significantly lower than those in the control group(P<0.05),and the triacylglycerol level was significantly higher than that in the control group(P<0.05).Hemorheology:the whole blood hyperviscosity,low shear viscosity,plasma viscosity and hematocrit in the experimental group were significantly higher than those
作者
佟鹏
王洋
梁瀛
Tong Peng;Wang Yang;Liang Ying(Department of Radiology,uludao Central Hospital,Huludao 125000,Liaoning Province,China;Nuclear Medicine,uludao Central Hospital,Huludao 125000,Liaoning Province,China;Catheter Room,Huludao Central Hospital,Huludao 125000,Liaoning Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2018年第32期5169-5174,共6页
Chinese Journal of Tissue Engineering Research
