摘要
【目的】明确池塘优势蓝藻对潜伏感染白斑综合征病毒(White spot syndrome virus,WSSV)凡纳滨对虾(Litopenaeus vannamei)的致死效应,为完善养殖对虾病害生态防控技术提供理论依据。【方法】分别设阳性对照组(PC)、阴性对照组(NC)、铜绿微囊藻(Microcystis aeruginosa)活藻组(MAL)和铜绿微囊藻死藻组(MAD),加藻组的铜绿微囊藻初始密度均为106cells/mL,每组各设3个平行,试验周期144 h。除NC组对虾不携带WSSV外,其他组的对虾均经WSSV人工注射感染。采用实时荧光定量PCR-TaqMan探针检测对虾样品中的WSSV携带状况,并通过显微计数法统计铜绿微囊藻细胞数量。【结果】NC组对虾在试验结束时(144 h)的累计死亡率为11.1%;PC、MAL和MAD组的对虾累计死亡率随时间推移呈明显升高趋势,至试验结束时的累计死亡率分别为64.4%、82.2%和88.9%,表现为加铜绿微囊藻(MAL和MAD)组的对虾累计死亡率远高于2个对照组(PC和NC)。MAL、MAD和PC组对虾肌肉样品均携带104copies/g以上的WSSV,其中又以MAD组的WSSV携带量最高。在铜绿微囊藻试验组中,对虾死亡率与铜绿微囊藻呈显著相关(P<0.05),与WSSV的相关性则未达显著水平(P>0.05)。【结论】铜绿微囊藻能有效加速WSSV感染对虾的死亡速率,增加对虾累计死亡率,即使是死亡的微囊藻细胞也能促进对虾体内WSSV的增殖。
【Objective】The lethal effects of dominant cyanophyta in pond to Litopenaeus vannamei infected with white spot syndrome virus(WSSV)were studied to offer theoretical reference for ecological technology of disease prevention and control in L.vannamei.【Method】Positive control group(PC),negative control group(NC),living Microcystis aeruginosa group(MAL)and dead M.aeruginosa group(MAD)were set and the initial density of M.aeruginosa in MAL and MAD was 106 cells/mL.Each group was set up three parallel experiments,with the trial period of 144 h.The tested L.vannamei in all groups were infected with WSSV by artificial injection except for the group NC.Quantitative real-time PCR was used to determine the viral load of WSSV in L.vannamei.The cell density of M.aeruginosa was evaluated using a hemocytometer.【Result】The cumulative mortality rate of L.vannamei in NC group was 11.1%at the end of the experiment(144 h).The cumulative mortality rate in the PC,MAL and MAD groups increased significantly over time,reaching 64.4%,82.2%and 88.9%respectively at the end of the trial.The cumulative mortality rate in the MAL and MAD groups were significantly higher than those in PC and NC groups.The muscle samples of MAL,MAD and PC groups all carried WSSV above 104 copies/g,among which the WSSV of MAD group was the highest.In treatment groups,the mortality rate of L.vannamei was significantly correlated with the cell density of M.aeruginosa(P<0.05),while the correlation with WSSV was not significant(P>0.05).【Conclusion】M.aeruginosa can effectively accelerate the mortality rate of L.vannamei infected by WSSV and increase the accumulative mortality rate.Even the dead algal cells can also promote the proliferation of WSSV in L.vannamei.
作者
曹煜成
徐煜
黄小帅
胡晓娟
李卓佳
李凡
陈勇贵
文国樑
CAO Yu-cheng;XU Yu;HUANG Xiao-shuai;HU Xiao-juan;LI Zhuo-jia;LI Fan;CHEN Yong-gui;WEN Guo-liang(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Research Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Key Laboratory of Guangdong Fishery Ecological Environment,Guangzhou 510300,China;South China Sea Biological Resources Development and Utilization Collaborative Innovation Center,SunYat-sen University,Guangzhou 510275,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2018年第8期1648-1653,共6页
Journal of Southern Agriculture
基金
现代农业(虾蟹)产业技术体系建设专项项目(CARS-48)
广东省渔港建设和渔业产业发展专项项目(A201701B06)
广东省科技计划项目(2017A040405001)
广东省渔业生态环境重点实验室开放基金项目(LFE-2015-14)
