摘要
为了探明混饲对吡喹酮在草鱼体内药动学参数的影响,分别采用口灌和静注不同的给药方式,在合适的时间点采集样品,经样品处理,采用超高效液相色谱仪进行检测,利用3P97药代动力学软件计算参数。结果显示:口灌吡喹酮原药时符合一级吸收二室开放模型,分布半衰期(t1/2α)、消除半衰期(t1/2β)、达峰时间(Tmax)、药时曲线下面积(AUC)和生物利用度(F)分别为1.2 h、18.1 h、0.4 h、19 885.8μg·h/L和47.0%。口灌吡喹酮和饲料混合物,其相应参数t1/2α、t1/2β、AUC和F分别为0.4 h、8.6 h、0.6 h、14 671.7μg·h/L和34.7%。通过比较发现口灌吡喹酮原型药物分布半衰期、消除半衰期、药时曲线下面积和生物利用度显著高于混饲,混饲吡喹酮达峰时间要明显高于口灌原形药物。
The samples were collected at different time points after oral and intravenous administrations to study the effect of mixed feeding on kinetic parameters of praziquantel(PZQ).The plasma samples were determined using ultra-high performance liquid chromatography after sample preparation.Afterwards,the pharmacokinetic parameters were estimated by 3P97 software.A two-compartment open model with first-order absorption was employed to describe the data obtained through oral treatment.Following oral treatment of PZQ,the results showed that the disposition half-life(t 1/2α),elimination half-life(t 1/2β),peak time(T max),area under the curve(AUC)and oral bioavailability(F)were 1.2 h,18.1 h,0.4 h,19 885.8μg·h/L and 47.0%,respectively.After oral treatment of PZQ and feed mixture,the corresponding parameters of t 1/2α,t 1/2β,T max,AUC and F were 0.4 h,8.6 h,0.6 h,14 671.7μg·h/L and 34.7%,respectively.The t 1/2α,t 1/2β,AUC and F after oral administration of PZQ were significantly higher than these parameters obtained after oral administration of PZQ and feed mixture.The peak time after oral treatment of PZQ was shorter than that of oral treatment of mixture.
作者
胥宁
杨移斌
刘永涛
董靖
杨秋红
艾晓辉
XU Ning;YANG Yi-bin;LIU Yong-tao;DONG Jing;YANG Qiu-hong;AI Xiao-hui(Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Hubei Freshwater Aquaculture Collaborative Innovation Center,Wuhan 430223,China)
出处
《淡水渔业》
CSCD
北大核心
2018年第5期73-78,共6页
Freshwater Fisheries
基金
中国水产科学研究院基本科研业务费专项课题(2017HY-ZC03
2016ZD1202)
