摘要
目的分析纤维蛋白原(FIB)与心肌缺血再灌注损伤(MIRI)早期炎症反应的相关性,探讨FIB对MIRI炎症反应的预测价值。方法纳入98例急性心肌梗死患者,对照组(即CAG组)仅行冠状动脉造影术(CAG),MIRI组(即PCI组)先后连续进行CAG和经皮冠脉成形术(PCI)。各组患者分别于术前(造影前)及术后2h采集患者静脉血,采用酶联免疫吸附法检测血浆FIB、肌酸激酶同工酶(CK-MB)、肌钙蛋白I(cTnI)以及肿瘤坏死因子α(TNF-α)、髓过氧化物酶(MPO)、巨噬细胞游走抑制因子(MIF)含量。根据血浆FIB水平将MIRI组分为3个血栓亚组,分析血浆FIB水平与炎症因子表达的相关性。结果 MIRI组患者血浆FIB、CK-MB、cTnI含量显著高于对照组(CAG组)(P<0.05或P<0.01),血浆炎症因子TNF-α、MPO、MIF含量明显升高(P<0.05)。FIB水平与炎症因子TNF-α、MPO、MIF水平存在一定相关性(r值分别是0.625,0.756,0.864,P<0.01)。结论血浆FIB水平与MIRI的炎症反应具有一定的相关性,FIB可作为心肌缺血再灌注相关炎症反应的预测因子。
Objective To investigate the correlation between fibrinogen(FIB)and early inflammatory response in patients with myocardial ischemia/reperfusion injury(MIRI)so as to explore the role of FIB in predicting early inflammation during MIRI.Methods Patients with acute myocardial infarction were treated by coronary angiography(CAG)and/or percutaneous coronary intervention(PCI)according to their conditions,and MIRI was induced by PCI.Blood samples were collected before and 2 hours after operation.FIB,creatine kinase-MB(CK-MB),troponin I(cTnI),tumor necrosis factor-α(TNF-α),myeloperoxidase(MPO)and macrophage migration inhibitory factor(MIF)in the plasma were measured by enzyme-linked immunosorbent assay.Patients subjected with PCI were divided into three subgroups according to the level of plasma FIB;the correlation between FIB and the above-mentioned inflammatory cytokines was evaluated in the patients subjected to MIRI.Results The levels of serum FIB,CK-MB and cTnI in the patients subjected to PCI were higher than those in patients subjected to CAG(P<0.05 or P<0.01).Compared with patients in CAG,inflammatory cytokines including TNF-α,MPO and MIF after PCI were significantly increased(P<0.05).The inflammatory cytokines presented a rising trend with the increase of FIB level,and there was a certain correlation between FIB and inflammatory cytokines(r=0.625,0.756,0.864,P<0.01 or P<0.001).Conclusion Serum inflammatory cytokines are up-regulated after MIRI.There is a certain correlation between FIB and inflammatory cytokines in patients with MIRI.FIB can be regarded as a predictor of inflammation associated with MIRI.
作者
许毛
刘娜
苏燕胜
秦小金
赵钰
马小亚
XU Mao;LIU Na;SU Yan-sheng;QIN Xiao-jin;ZHAO Yu;MA Xiao-ya(Department of Pharmacy,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004;Department of Urology,Hospital 323 of People s Liberation Army,Xi'an 710054;Department of Cardiology,The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2018年第5期698-702,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
国家自然科学基金资助项目(No.81400272)
陕西省青年科技新星计划项目(No.2016KJXX-20)
西安交通大学第二附属医院科研基金重点项目[No.YJ(ZD)201310]
西安交通大学第二附属医院人才培养专项科研基金资助项目[No.RC(XM)201202]~~
