摘要
为了解山东省滕州市羊隐孢子虫感染情况和种类,从该市2个绵羊场、2个山羊场采集222份羊粪便样品,提取所有样品的基因组DNA,采用巢式PCR扩增隐孢子虫actin基因,对阳性样品进行序列测定和分析。结果显示,滕州市羊隐孢子虫总的感染率为6.76%,其中绵羊7.84%,山羊5.83%;不同月龄的羊感染情况不同,6~12月龄绵羊感染率最高(20.69%),而12月龄以上的山羊感染率最高(10.00%);共发现3种隐孢子虫感染,其中微小隐孢子虫(Cryptosporidium parvum,C.parvum)检出率最高为53.33%,其次是肖氏隐孢子虫(C.xiaoi)为40.00%,仅发现1例(6.67%)泛在隐孢子虫(C.ubiquitum)感染;序列比对结果显示,本试验所获得基因序列与Gen Bank中的C.parvum、C.xiaoi、C.ubiquitum actin基因序列同源性达到100%;系统进化分析显示,滕州市的羊样品中鉴定的actin序列,与Gen Bank中的相应虫种序列在同一分支上。研究结果为深入了解滕州市羊隐孢子虫流行情况及制定有效的防控措施提供了依据。
In order to understand infection status and species distribution of Cryptosporidium in sheep and goats,222 fecal samples were collected from Tengzhou city of Shandong province.The DNA materials were extracted and amplified in nested-PCR targeting Cryptosporidium actin gene.The PCR positive samples were sequenced for identification of Cryptosporidium species.The infection rates were 7.84%for sheep and 5.83%for goats and varied with different host ages.Additionally,6-12 month old sheep had the highest infection rate at,20.69%while>12 month old goats were the most susceptible group at 10.00%.Three Cryptosporidium species were identified.Cryptosporidium parvum(53.33%)was the most prevalent species,followed by C.xiaoi(40.00%)and C.ubiquitum(6.67%).BLAST analysis demonstrated that these sequences had 100%identity to the reported corresponding Cryptosporidium sequences in GenBank.These results provided valuable information on the current prevalence and building up strategy for prevention and control of Cryptosporidium infection in sheep and goat populations.
作者
朱维
米荣升
王进香
王翠
黄燕
张烨华
贾海燕
张晓丽
韩先干
陈兆国
ZHU Wei;MI Rong-sheng;WANG Jin-xiang;WANG Cui;HUANG Yan;ZHANG Ye-hua;JIA Hai-yan;ZHANG Xiao-li;HAN Xian-gan;CHEN Zhao-guo(Tengzhou Animal Husbandry and Veterinary Technology Service Center of Shandong Province,Tengzhou 277599,China;Key Laboratory of Animal Parasitology of Ministry of Agriculture,Laboratory of Quality and Safety Risk Assessment for Animal Products on Biohazards(Shanghai)of Ministry of Agriculture,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Ningxia Center for Animal Disease Control and Prevention,Yinchuan 750011,China;Henan Center for Animal Disease Control and Prevention,Zhengzhou 450008,China))
出处
《中国动物传染病学报》
CAS
北大核心
2018年第4期69-75,共7页
Chinese Journal of Animal Infectious Diseases
基金
宁夏回族自治区科技支撑计划[宁农科(2016)1号]
上海科技兴农重点攻关项目课题(沪农科攻字(2015)第1-10号)
国家农产品质量安全风险评估项目(GJFP201800703)
中央级公益性科研院所基本科研业务费专项资金项目(2016JB13)
中国农业科学院科技创新工程
