摘要
目的:建立克雷伯杆菌肺炎致多器官功能障碍小鼠模型。方法:将KM小鼠随机分为对照组、模型组,每组32只;采用气管插管法,模型组注入肺炎克雷伯杆菌,对照组注入相同剂量的生理盐水;分别于造模后第1、3、5、7天每组处死8只小鼠,动态观察小鼠症状、体征变化、CK-MB、CK-M、ALT、AST水平,肺组织TNF-α、IL-6水平及肺脏、心脏、肝脏组织病理变化。结果:模型组小鼠于感染后第1天、第3天症状、体征病态改变较明显,CKMB、CKM、ALT含量,肺组织TNF-α、IL-6水平较对照组明显升高,尤以第1天更为明显。模型组小鼠肺脏、心脏、肝脏组织病理改变在不同感染时相均较对照组加重,以感染后第1天、第3天病理损伤较明显。结论:采用肺炎克雷伯杆菌气管滴入法制备多器官功能障碍模型,制备方法简单,便于复制,适用于细菌性肺炎导致的多器官功能障碍发病机制和临床药效学的研究。
Objective:Establish Klebsiella pneumoniae-induced multiple organ dysfunction mouse model.Methods:KM mice were randomly divided into control group and model group with 32 animals in each group;using tracheal intubation,Klebsiella pneumoniae was injected into the model group,and the same dose of physiological saline was injected into the control group;eight mice were sacrificed on the 1st,3rd,5th,and 7th days after modeling respectively;dynamic observation of mouse symptoms,signs,CKMB,CKM,ALT,AST levels and pathological changes of lung,heart,liver.Results:The mice in the model group had more obvious pathology changes in symptoms and signson the first and third day after infection,the levels of CKMB,CKM,and ALT were significantly higher than those in the control group,especially on the first day;the pathological changes of the lung,heart and liver in the model group were aggravated at different infection phases compared with the control group,the pathological lesions on the first and third day after infection were more obvious.Conclusion:Multiple organ dysfunction models were prepared using Klebsiella pneumoniae tracheal instillation method.The preparation method is simple and easy to replicate.It is applicable to the pathogenesis and clinical pharmacodynamics of multiple organ dysfunction caused by bacterial pneumonia.
作者
李猛
王志梅
徐红日
王成祥
马战平
Li Meng;Wang Zhimei;Xu Hongri(Shaanxi Provincial Hospital of Traditional Chinese Medicine,Xi’an 710003)
出处
《陕西医学杂志》
CAS
2018年第9期1099-1104,共6页
Shaanxi Medical Journal
基金
陕西省自然科学基金资助项目(2013JC2-08)
陕西省科技厅重点研发计划项目(2017ZDXM-SF-109)
西安市科技局计划项目(SF1419-2)
陕西省中医药管理局科研项目(13-LC118)
