摘要
为研究不同葡萄糖酸铬添加量对锦鲤Cyprinus carpio血清中激素水平和肝胰脏中、糖代谢相关酶活性的影响,试验配制7种饲料,其中Cr^(3+)添加量分别为0(对照)、0.1、0.2、0.4、0.8、1.6、3.2 mg/kg(饲料),选择初始体质量为(4.18±0.38)g的锦鲤525尾,随机分为7组,每组设3个重复,每个重复放25尾鱼,投饲养殖周期为8周。结果表明:在饲料中添加葡萄糖酸铬能显著降低锦鲤血清中皮质醇(COR)含量(P<0.05),Cr^(3+)添加量为0.8 mg/kg时能显著提高锦鲤胰岛素(INS)水平(P<0.05),胰岛素受体(ISR)水平在Cr^(3+)添加量≥0.4 mg/kg时显著高于对照组和0.1 mg/kg组(P<0.05),而不同Cr^(3+)添加量未对胰高血糖素(GC)造成影响(P>0.05);饲料中Cr^(3+)添加量≥0.2 mg/kg时,能显著提高锦鲤肝胰脏中葡萄糖激酶(GK)活性(P<0.05),显著降低葡萄糖-6-磷酸酶(G6P)活性(P<0.05);Cr^(3+)添加量为0.1~1.6 mg/kg时,能显著提高丙酮酸激酶(PK)活性(P<0.05),显著降低磷酸烯醇式丙酮酸羧基酶(PEPCK)活性(P<0.05);Cr^(3+)添加量为0.4~0.8 mg/kg时,能显著提高葡萄糖-6-磷酸脱氢酶(G6PDH)活性,Cr^(3+)添加量≥0.8 mg/kg时,琥珀酸脱氢酶(SDH)活性显著升高(P<0.05);除Cr^(3+)添加量为3.2 mg/kg组锦鲤苹果酸脱氢酶(MDH)活性显著高于对照组(P<0.05)外,其余各组间均无显著性差异(P>0.05)。研究表明,饲料中添加葡萄糖酸铬可有效提高锦鲤对饲料中糖类物质的利用能力,建议饲料中Cr^(3+)的适宜添加量为0.8 mg/kg。
A 8-week feeding trial was conducted to evaluate the effects of different dietary chromium gluconate levels on serum hormone levels and glyco-metabolism-related enzymes activities in hepatopancreas of koi carp Cyprinus carpio.Juvenile koi carp with body weight of(4.18±0.38)g were reared in a plastic 300 L tanks and fed diets containing chromium gluconate at a Cr^3+dose of 0(control group),0.1,0.2,0.4,0.8,1.6,and 3.2 mg/kg with triplication.The results showed that the fish fed diets containing chromium gluconate had significantly lower serum cortisol(COR)concentration than the fish fed the control diet did(P<0.05),especially significantly higher insulin level in fish fed 0.8 mg/kg chromium gluconate diet than that in the fish fed the control diet and 0.1 mg/kg chromium gluconate diet(P<0.05),without significant difference in glycogen(GC)level in the fish fed diet containing chromium gluconate and the control diet(P>0.05).The fish fed diets containing≥0.2 mg/kg chromium gluconate had significantly higher glucokinase(GK)and lower glucose-6-phosphatase(G6P)activities than those fed the control diet did(P<0.05).The diets containing 0.1-1.6 mg/kg chromium gluconate led to significant increase in pyruvate kinase(PK)activities and to significant decrease in phosphoenolpyruvate carboxylase activities(PEPCK)(P<0.05).There were significantly higher activities of glucose 6-phosphatase dehydrogenase(G6PDH)in 0.4-0.8 mg/kg groups than that in the fish fed control diet(P<0.05).Significantly higher succinic acid dehydrogenase(SDH)activity was observed in the fish fed diet containing≥0.8 mg/kg chromium gluconate,significantly higher SDH activity than that in the control group(P<0.05),without significant difference among the other groups(P>0.05),except for Cr^3+dose of 3.2 mg/kg.In conclusion,the dietary chromium gluconate shows significant impact on the carbohydrate utilization in juvenile koi carp and 0.8 mg/kg is recommended to be the optimal level under this experimental conditions.
作者
崔培
王景倩
程镇燕
牟希东
乔秀亭
孙金辉
CUI Pei;WANG Jing-qian;CHENG Zhen-yan;MU Xi-dong;QIAO Xiu-ting;SUN Jin-hui(Key Laboratory of Tropical&Subtropical Fishery Resource Application and Cultivation,Ministry of Agriculture,Guangzhou 510380,China;College of Fisheries Science,Tianjin Key Laboratory of Aqua-ecology and Aquaculture,Tianjin Agricultural University,Tianjin 300384,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第4期481-486,共6页
Journal of Dalian Ocean University
基金
农业部热带亚热带水产资源利用与养殖重点实验室开放基金资助项目
浙江省重中之重一级学科(水产)开放基金资助项目
天津市自然科学基金资助项目(14JCQNJC15100)
关键词
锦鲤
葡萄糖酸铬
血清激素
糖代谢酶活性
Cyprinus carpio
chromium gluconate
serum hormone
glyco-metabolism-related enzyme