摘要
目的了解腹泻患儿临床分离沙门菌株的遗传基因型及耐药特征。方法收集2014年7月至2015年10月腹泻患儿大便标本,经大便培养分离鉴定沙门菌43株。用多位点序列分型技术(MLST)对43株沙门菌的靶基因进行PCR扩增和测序,通过eBURST方法对其进行进化聚类分析,并通过STAT2软件进行菌株基因遗传进化分析;检测菌株对临床常用抗生素的耐药性。结果 MLST分析显示,43株沙门菌可分为17个序列类型(ST),未发现新的型别;其中ST11和ST 34是优势ST型别,分别占32.56%、23.26%。43株沙门菌对阿莫西林/克拉维酸、头孢噻肟、头孢他啶和头孢吡肟的耐药率分别为13.95%、27.91%、16.28%和11.63%,未发现哌拉西林/他唑巴坦、亚胺培南耐药菌株。结论沙门菌感染的腹泻患儿以ST11和ST34为优势克隆株,对三、四代头孢菌素均出现耐药,对哌拉西林/他唑巴坦或亚胺培南仍敏感。
Objective To explore the genetic phenotypes of Salmonella isolates in clinical specimens of children with diarrhea and to clarify the drug resistance characteristics of Salmonella.Method Stool specimens were collected in diarrhea children from July 2014 to October 2015,and 43 strains of Salmonella were isolated.The target gene of 43 Salmonella strains was amplified and sequenced by multilocus sequence typing(MLST).The genetic clustering analysis was performed by eBURST method,and the genetic evolution of bacterial genes was analyzed by STAT2 software.The resistance of Salmonella strains to the commonly used antibiotics was tested.Results MLST analysis showed that 43 strains of Salmonella could be divided into 17 sequence types(ST)and no new type was found,among which ST11 and ST34 were the dominant ST types,accounting for 32.56%and 23.26%respectively.The rates of 43 Salmonella strains resistant to amoxicillin/clavulanic acid,cefotaxime,ceftazidime and cefepime were 13.95%,27.91%,16.28%and 11.63%respectively.The strains resistant to piperacillin/tazobactam and imipenem were not found.Conclusion ST11 and ST34 were the dominant clones of Salmonella strains isolated from these diarrhea children,which was resistant to three-or four-generation cephalosporins,while they were still sensitive to piperacillin/tazobactam or imipenem.
作者
徐飞
王庭庭
谈华
李玫
金玉
郭红梅
XU Fei;WANG Tingting;TAN Hua;LI Mei;JIN Yu;GUO Hongmei(Children's Hospital Affiliated to Nanjing Medical University,Nanjing 210008,Jiangsu,China)
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2018年第7期520-523,共4页
Journal of Clinical Pediatrics
基金
国家临床重点专科建设项目资助(No.2011873)
关键词
腹泻
沙门菌
多位点序列分型
细菌耐药性检测
儿童
diarrhea
Salmonella
multilocus sequence typing
bacterial resistance detection
child
