摘要
【目的】以艳花独蒜兰(Pleione aurita)、云南独蒜兰(P.yunnanensis)和黄花独蒜兰(P.forrestii)的休眠假鳞茎为试验材料,研究低温贮藏期间独蒜兰芽发育和生理生化的变化,为独蒜兰属植物的低温贮藏提供参考。【方法】将3种独蒜兰贮藏在(4±1)℃条件,在0、30、60、90、120 d时测定新芽长度、可溶性蛋白含量、SOD活性和POD活性。【结果】3种独蒜兰假鳞茎在(4±1)℃低温冷藏60 d后芽开始伸长。艳花独蒜兰假鳞茎中可溶性蛋白含量无明显变化,SOD活性在处理120 d时出现显著降低,POD活性在30~60 d时呈现明显下降,90~120 d再上升。云南独蒜兰和黄花独蒜兰假鳞茎可溶性蛋白呈增加趋势,而SOD活性无明显变化,POD活性在0~30 d时明显下降,30~60 d呈上升趋势。【结论】艳花独蒜兰、云南独蒜兰和黄花独蒜兰落叶休眠假鳞茎在(4±1)℃条件贮藏60 d后可进行栽培,POD活性的降低有利于三者新芽的萌动。
[Purpose]In order to provide a reference for the cold storage of Pleione species,the dormant pseudobulbs of P.aurita,P.yunnanensis,and P.forrestii were used as materials to investigate the bud development and physiology during the cold storage.[Method]The pseudobulbs were stored at(4±1)℃,the lengths of new bud were measured,as well as the soluble protein content,SOD and POD activity after 0,30,60,90,120 days.[Result]The buds started to germinate after 60 days.For P.aurita,the soluble protein content decreased slightly,while SOD activity declined significantly on 120 day.POD activity declined from 30 to 60 days,and then increased from 90 to 120 days.The soluble protein content of P.yunnanensis and P.forrestii increased during this period,while the change of SOD activity was not significant.The POD activity declined from 0 to 30 days,and then increased from 30 to 60 days.[Conclusion]After stored at(4±1)℃for 60 days,the dormant pseudobulbs of P.aurita,P.yunnanensis,and P.forrestii should be planted.The decreased of POD activity promoted the germination of new bud.
作者
江鸣涛
李云霞
张艺祎
彭东辉
兰思仁
吴沙沙
JIANG Mingtao;LI Yunxia;ZHANG Yiyi;PENG Donghui;LAN Siren;WU Shasha(College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Cross-Straits Orchids Conservation and Research Center,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第3期572-576,共5页
Journal of Yunnan Agricultural University:Natural Science
基金
国家教育部博士点基金(20133515120017)
福建省自然科学青年基金(2014J0102)
福建省教育厅新教师项目(JA13123)
关键词
低温贮藏
休眠
生理
芽
cold storage
dormancy
physiology
bud
