摘要
目的比较非小细胞肺癌患者与正常体检人群外周血相对端粒长度的差异,同时观察端粒长度与非小细胞肺癌临床特征的相关性。方法运用荧光实时定量PCR法测定正常人群与非小细胞肺癌组外周血端粒长度,并进行统计学比较,同时分析肺癌组端粒长度与临床分期、肿瘤大小、吸烟史、年龄的关联性。结果肺癌组端粒长度较正常人群明显缩短,差异有统计学意义(P<0.05);端粒长度与肿瘤大小、年龄、吸烟史呈负相关,肿瘤越大,端粒长度越短,年龄越大,端粒长度越短,吸烟较不吸烟人群端粒长度缩短,差异有统计学意义(P<0.05)。结论非小细胞肺癌组的端粒长度较健康对照组明显缩短,有望成为非小细胞肺癌早期诊断的生物标志物之一;端粒长度与非小细胞肺癌的临床分期、病理类型、恶性程度密切相关,有望作为新的肿瘤标记物或药物治疗靶点。
Objective To compare the difference of relative telomere length in peripheral blood between patients with non-small cell lung cancer and normal people,and to observe the correlation between telomere length and clinical features of non-small cell lung cancer.Methods The telomere length in peripheral blood of normal people and nonsmall cell lung cancer group was measured by fluorescence real-time quantitative PCR and statistically compared.At the same time,the correlation between telomere length and clinical stage,tumor size,smoking history and age in lung cancer group was analyzed.Results Telomere length in lung cancer group was significantly shorter than that in normal group(P<0.05).Telomere length was negatively correlated with tumor size,age and smoking history.The larger the tumor,the shorter the telomere length.The older the age,the shorter the telomere length.The telomere length of smokers was shorter than that of nonsmokers,and the difference was statistically significant(P<0.05).Conclusion Telomere length in the non-small cell lung cancer group is significantly shorter than that in the healthy control group,which is expected to become one of the biomarkers for early diagnosis of non-small cell lung cancer.Telomere length is closely related to clinical stage,pathological type and malignancy degree of non-small cell lung cancer,and is expected to be a new tumor marker or drug treatment target.
作者
石万萍
李桂香
SHI Wanping;LI Guixiang(Department of Gamma Knife,Second Hospital,Lanzhou University,Lanzhou 730000,China)
出处
《中国医药科学》
2018年第11期9-12,149,共5页
China Medicine And Pharmacy
关键词
非小细胞肺癌
端粒长度
靶向治疗
临床分期
Non-small cell lung cancer
Telomere length
Targeted therapy
Clinical stage
