摘要
为探讨干旱胁迫下烟草脯氨酸杂种优势的分子遗传基础,以抗旱性差异较大的7个烟草品种及其组配的10个杂交组合为材料,采用盆栽试验设置干旱胁迫处理,进行了烟草脯氨酸杂种优势及其相关基因差异表达分析。结果表明,干旱胁迫下烟草脯氨酸含量在胁迫早期持续增加,第14天后开始下降;杂交组合间脯氨酸含量性状杂种优势差异明显,中亲优势最高40.35、最低-51.66;强优势组合中脯氨酸合成关键酶基因P5CS和δ-OAT相对表达量明显高于弱优势组合,分别是弱优势组合的2.10倍、1.87倍;脯氨酸杂种优势与P5CS基因相对表达量在旺长期持续干旱7 d时存在显著正相关关系,与δ-OAT基因相对表达量在伸根期持续干旱14 d、旺长期持续干旱7 d和14 d呈显著或极显著的相关关系,相关系数分别为0.93、0.98和-0.96。结果显示,脯氨酸合成关键酶基因δ-OAT和P5CS的时序性上调或下调表达是相应时期烟草脯氨酸含量性状杂种优势形成的分子基础。
In order to explore the molecular genetic basis of proline heterosis in tobacco under drought stress,7 tobacco varieties with different drought resistance and 10 hybrid combinations were used as materials,and drought stress treatments were set up to analyze proline heterosis and differential expression of related genes in tobacco by pot experiments.The results showed that the content of tobacco proline under drought stress increased continuously at the early stage and decreased after the 14th day.The heterosis of proline content in hybrid combinations was significantly different,and the proline content had the mid-parent heterosis with the highest value of 40.35 and the lowest of-51.66.The relative expression levels of the proline synthesis key enzyme genes P5CS andδ-OAT were significantly higher in the strong-dominant combinations,being 2.10 times and 1.87 times of those of the weak-dominant combinations,respectively.There was a significant positive correlation between the proline heterosis and P5CS gene relative expression after persistent drought for 7d at the vigorous growth stage.The correlations between the proline heterosis andδ-OAT gene relative expression were significant or extremely significant after persistent drought for 14d at the root extension stage and persistent drought for 7d and 14d at the vigorous growth,with correlation coefficients of 0.93,0.98 and-0.96,respectively.Therefore,the up-regulation or down-regulation ofδ-OAT and P5CS was the molecular basis for the formation of heterosis of tobacco proline content during the corresponding growth periods.
作者
孟军
吴迪
夏志林
喻奇伟
王军
刘双
陈倩
田茂竹
李显航
汪志威
刘仁祥
MENG Jun;WU Di;XIA Zhilin;YU Qiwei;WANG Jun;LIU Shuang;CHEN Qian;TIAN Maozhu;LI Xianhang;WANG Zhiwei;LIU Renxiang(Guizhou Key Laboratory for Tobacco Quality Research,Guiyang 550025,China;Zunyi Tobacco Company,Zunyi,Guizhou 564000,China;Bijie Tobacco Company,Bijie,Guizhou 551700,China)
出处
《中国烟草科学》
CSCD
北大核心
2018年第2期1-7,共7页
Chinese Tobacco Science
基金
贵州省科技厅人才团队建设项目“贵州省烟草品质遗传改良与生物转化科技创新人才团队”{黔科合人才团队[2015]4011号},贵州省高层次创新型人才培养计划--百层次人才{黔科合平台人才[2016]5663号}
贵州省烟草公司重大专项“优质烤烟品种选育关键技术研究与品种选育”(黔烟科201602)
贵州省烟草公司遵义市公司项目“遵义烟区烤烟品种筛选布局与后备品种选育研究”{遵烟计[2016]07号}
关键词
烟草
干旱胁迫
脯氨酸
杂种优势
基因差异表达
Nicotiana tabacum
drought stress
proline
heterosis
gene differential expression
