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局部应用降钙素基因相关肽对小鼠肩袖损伤后早期愈合的影响 被引量:4

Local administration of calcitonin gene-related peptide promotes early healing after rotator cuff injury in mice
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摘要 背景:骨腱界面的损伤修复一直是骨科和运动医学领域里的一大难题,新生骨的形成有利于骨腱界面的愈合。降钙素基因相关肽在维持组织稳态和组织损伤后修复过程中发挥着非常重要的作用,但是其在骨腱界面损伤中的作用至今未见报道。目的:建立小鼠肩袖损伤模型,观察降钙素基因相关肽对损伤处成骨因子骨钙素表达的影响,评估其对小鼠肩袖损伤后早期愈合的作用。方法:建立小鼠冈上肌止点-肱骨损伤模型,随机分成2组:实验组术后当天经盂肱关节注入5nmol/kg降钙素基因相关肽,每周3次,共2周;对照组注入等量的生理盐水。术后4,6周安乐死动物获取肩袖损伤标本,行苏木精-伊红染色观察其组织病理变化,实时荧光定量PCR检测骨钙素mRNA的表达,免疫组织化学、免疫印迹实验观察骨钙素的蛋白表达水平,生物力学检测两组肩袖损伤标本的拉断载荷。结果与结论:术后4,6周实验组成骨因子骨钙素的mRNA及蛋白表达水平均高于对照组(P<0.05);与对照组比较,实验组术后4周有更多的纤维软骨生成,术后6周有更多的新生骨生成;实验组术后4周的力学拉断载荷比对照组轻微上升,但没有统计学差异(P>0.05);术后6周,实验组的力学拉断载荷比对照组高(P<0.05)。结果证实局部注射降钙素基因相关肽能提高损伤处骨钙素的表达及新生骨的形成,有利于肩袖损伤后早期愈合。 BACKGROUND:Repair of tendon interface is a difficulty in orthopedics and sports medicine,and the formation of new bone is conducive to its healing.Calcitonin gene-related peptide(CGRP)plays a critical role in tissue homeostasis and repair,but its effect on the bone-tendon interface repair is unknown.OBJECTIVE:To investigate the effect of CGRP on the expression of osteocalcin and to evaluate the effect of CGRP on the early healing of rotator cuff injury in a mouse model.METHODS:A mouse model of supraspinatus insertion-humerus injury was created.All model mice were then randomized into two groups,and given the injection of 5 nmol/kg CGRP(experimental group)or same volume of normal saline(control group)through the glenohumeral joint immediately after operation,thrice weekly,for 2 weeks.The mice were sacrificed at postoperative 4 and 6 weeks to remove the rotator cuff samples for hematoxylin-eosin staining and biomechanical test.The mRNA and protein expression levels of osteocalcin were surveyed by qRT-PCR,immunohistochemistry,and western blot assay.RESULTS AND CONCLUSION:At postoperative 4 and 6 weeks,the mRNA and protein expression of osteocalcin in the experimental group were significantly higher than those in the control group(P<0.05).Compared with the control group,there were more fibrocartilages in the experimental group at 4 weeks postoperatively,and more new bone formation in the experimental group at 6 weeks postoperatively.At 4 weeks postoperatively,failure load in the experimental group increased slightly,but it was not significantly different from that in the control group(P>0.05);at 6 weeks postoperatively,failure load in the experimental group was significantly higher than that in the control group(P<0.05).To conclude,local injection of CGRP can up-regulate the expression of osteocalcin and the formation of new bone at the injury site,which can enhance early healing the injured rotator cuff.
作者 陈洋 王占稳 陈灿 吕红斌 Chen Yang;Wang Zhan-wen;Chen Can;Lv Hong-bin(Department of Sports Medicine and Research Center of Sports Medicine,Xiangya Hospital,Central South University,Changsha 410008,Hunan Province,China;Key Laboratory of Organ Injury,Aging and Regenerative Medicine of Hunan Province,Xiangya Hospital,Central South University,Changsha 410008,Hunan Province,China;Xiangya Hospital of Central South University-International Chinese Musculoskeletal Research Society Collaborating Center for Sports Medicine,Xiangya Hospital,Central South University,Changsha 410008,Hunan Province,China)
出处 《中国组织工程研究》 CAS 北大核心 2018年第12期1859-1865,共7页 Chinese Journal of Tissue Engineering Research
基金 国家自然科学基金重点项目(81730068) 国家自然科学基金面上项目(81472072) 中南大学研究生科研创新项目(2016zzts532)~~
关键词 肩袖损伤 降钙素基因相关肽 骨钙素 动物模型 拉断载荷 组织构建 肩关节 腱损伤 模型 动物 降钙素基因相关肽 骨钙素 Shoulder Joint Models,Animal Calcitonin Gene-Related Peptide Osteocalcin
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